水稻包穗突变体sui2的遗传分析和基因精细定位

孙琦, 赵志超, 张瑾晖, 张锋, 程治军, 邹德堂

Genetic analysis and fine mapping of a sheathed panicle mutant sui2 in rice (Oryza sativa L.)

Qi SUN, Zhi-Chao ZHAO, Jin-Hui ZHANG, Feng ZHANG, Zhi-Jun CHENG, De-Tang ZOU

图3 候选基因SUI2基因的图位克隆示意图 A: SUI2初步定位在4号染色体标记S4-14和S4-15之间; B: SUI2被精细定位在S4-14.1和S4-14.2之间的110 kb范围内; C: SUI2精细定位区间内包含的14个基因和2个转座子。横线的上方为定位所用的分子标记, 横线下方为交换单株数。

Fig. 3 Schematic diagram of map-based cloning on the candidate gene SUI2 A: Rough mapping of the gene SUI2 flanked by two markers S4-14 and S4-15; B: SUI2 was fine mapped to a 110 kb interval delimited by two markers S4-14.1 and S4-14.2 region; C: 14 ORFs and 2 transposons within the fine-mapped fragment. The molecular markers used in linkage analysis were indicated above the bold lines, and the umbers beneath the bold lines represented the recombinants identified by the corresponding markers.