小麦高效转基因受体品系CB037的抗条锈性分析

郑燕燕, 黄德华, 李金龙, 张会飞, 鲍印广, 倪飞, 吴佳洁

Analysis of the stripe rust resistance in a wheat line CB037 with high regeneration and transformation efficiency

Yan-Yan ZHENG, De-Hua HUANG, Ji-Long LI, Hui-Fei ZHANG, Yin-Guang BAO, Fei NI, Jia-Jie WU

表1 本研究开发的分子标记

Table 1 Molecular markers developed in this study

分子标记 Molecular marker 染色体及位置 Chromosome & position (Mb) 正向引物 Forward primer (5′-3′) 反向引物 Reverse primer (5′-3′) 退火温度 Annealing temperature (℃) 内切酶 Restriction enzyme 多态性 Polymorphism (bp) CBSR-53/54 a 1B, 69.9 GAACGGCTCGGATCTGATC TGTACAACAACAAGAAGGAGGAG 55 Dde I CBS: 79+83 CBR: 163 CBSR-1/2 b 1B, 98.7 CCGGAGTTGAAGATAAGTAGTTG CTTCCCTGTATTCGAACAGACTCA 56 N/A CBS: 242 CBR: 0 CBSR-35/36 a 1B, 115.3 ACACCGAGGTGCGTCTAAT TATCCCATGCGGTAAATGGAC 55 Msp I CBS: 150+57 CBR: 207 InDel-3/4 b 1B, 376.6 TCCATGGCAGTACCGCTTGCTT CCGGAGCAGCAATGAAACGTC 56 N/A CBS: 240 CBR: 207 CBSR-25/26 b 4A, 723.7 GCCGCGTTTTGCATGTTTTCCG TGGGTTGAATTTTGGGTTGTTTGAA 56 N/A CBS: 408 CBR: 378