小麦高效转基因受体品系CB037的抗条锈性分析
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郑燕燕, 黄德华, 李金龙, 张会飞, 鲍印广, 倪飞, 吴佳洁
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Analysis of the stripe rust resistance in a wheat line CB037 with high regeneration and transformation efficiency
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Yan-Yan ZHENG, De-Hua HUANG, Ji-Long LI, Hui-Fei ZHANG, Yin-Guang BAO, Fei NI, Jia-Jie WU
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表1 本研究开发的分子标记
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Table 1 Molecular markers developed in this study
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分子标记 Molecular marker | 染色体及位置 Chromosome & position (Mb) | 正向引物 Forward primer (5′-3′) | 反向引物 Reverse primer (5′-3′) | 退火温度 Annealing temperature (℃) | 内切酶 Restriction enzyme | 多态性 Polymorphism (bp) | CBSR-53/54 a | 1B, 69.9 | GAACGGCTCGGATCTGATC | TGTACAACAACAAGAAGGAGGAG | 55 | Dde I | CBS: 79+83 CBR: 163 | CBSR-1/2 b | 1B, 98.7 | CCGGAGTTGAAGATAAGTAGTTG | CTTCCCTGTATTCGAACAGACTCA | 56 | N/A | CBS: 242 CBR: 0 | CBSR-35/36 a | 1B, 115.3 | ACACCGAGGTGCGTCTAAT | TATCCCATGCGGTAAATGGAC | 55 | Msp I | CBS: 150+57 CBR: 207 | InDel-3/4 b | 1B, 376.6 | TCCATGGCAGTACCGCTTGCTT | CCGGAGCAGCAATGAAACGTC | 56 | N/A | CBS: 240 CBR: 207 | CBSR-25/26 b | 4A, 723.7 | GCCGCGTTTTGCATGTTTTCCG | TGGGTTGAATTTTGGGTTGTTTGAA | 56 | N/A | CBS: 408 CBR: 378 |
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