小麦高效转基因受体品系CB037的抗条锈性分析
郑燕燕, 黄德华, 李金龙, 张会飞, 鲍印广, 倪飞, 吴佳洁

Analysis of the stripe rust resistance in a wheat line CB037 with high regeneration and transformation efficiency
Yan-Yan ZHENG, De-Hua HUANG, Ji-Long LI, Hui-Fei ZHANG, Yin-Guang BAO, Fei NI, Jia-Jie WU
表1 本研究开发的分子标记
Table 1 Molecular markers developed in this study
分子标记
Molecular marker
染色体及位置
Chromosome & position (Mb)
正向引物
Forward primer
(5′-3′)
反向引物
Reverse primer
(5′-3′)
退火温度
Annealing temperature (℃)
内切酶
Restriction enzyme
多态性
Polymorphism
(bp)
CBSR-53/54 a 1B, 69.9 GAACGGCTCGGATCTGATC TGTACAACAACAAGAAGGAGGAG 55 Dde I CBS: 79+83
CBR: 163
CBSR-1/2 b 1B, 98.7 CCGGAGTTGAAGATAAGTAGTTG CTTCCCTGTATTCGAACAGACTCA 56 N/A CBS: 242
CBR: 0
CBSR-35/36 a 1B, 115.3 ACACCGAGGTGCGTCTAAT TATCCCATGCGGTAAATGGAC 55 Msp I CBS: 150+57
CBR: 207
InDel-3/4 b 1B, 376.6 TCCATGGCAGTACCGCTTGCTT CCGGAGCAGCAATGAAACGTC 56 N/A CBS: 240
CBR: 207
CBSR-25/26 b 4A, 723.7 GCCGCGTTTTGCATGTTTTCCG TGGGTTGAATTTTGGGTTGTTTGAA 56 N/A CBS: 408
CBR: 378