基于BSA-Seq技术挖掘大豆中黄622的多小叶基因

张之昊, 王俊, 刘章雄, 邱丽娟

Mapping of an incomplete dominant gene controlling multifoliolate leaf by BSA-Seq in soybean (Glycine max L.)

ZHANG Zhi-Hao, WANG Jun, LIU Zhang-Xiong, QIU Li-Juan

表3 引物序列和信息

Table 3 Sequence and information of the primers

引物名称 Primer ID 上游引物序列 Forward primer (5°-3°) 下游引物序列 Reverse primer (5°-3°) 产物大小 Product size (bp) 检测位点数目 Number of detection sites SNP 1 GCCGTAGCACTTCACTCATTCA ATGTTCTATAAGCCGTTTTTTGGAAG 5633 5 SNP 2 TCTACTCGCAAAAGGCACAG CATTTATATGTTCGTCAATATTTAG 7016 1 SNP 3 GGAATGCCAATGGGCTTTAATT GATCTTGCTTACCAATTTCTCG 1247 3