基于BSA-Seq技术挖掘大豆中黄622的多小叶基因

张之昊, 王俊, 刘章雄, 邱丽娟

Mapping of an incomplete dominant gene controlling multifoliolate leaf by BSA-Seq in soybean (Glycine max L.)

ZHANG Zhi-Hao, WANG Jun, LIU Zhang-Xiong, QIU Li-Juan

图2 利用2种关联方法鉴定多小叶基因候选区间 A: ED关联分析结果, 横坐标为染色体位置, 纵坐标代表拟合后的欧式距离(ED)值的5次方, 黑色的线为拟合后的ED的5次方作关联值, 红色的虚线代表显著性关联阈值。B: SNP-index关联分析结果, 横坐标为染色体位置, 黑色的线为拟合后的ΔSNP-index值, 红色的线代表置信度为0.99的阈值线, 蓝色的线代表置信度为0.95的阈值线, 绿色的线代表置信度为0.90的阈值线。2种关联分析结果均表明, 与多小叶相关的关联区域位于11号染色体末端。

Fig. 2 Identification of multiple leaflet gene candidate intervals using two association methods A: ED correlation analysis results, the abscissa is the chromosome position, the ordinate represents the fifth power of the euclidean distance (ED) value after fitting, the black line is the fifth power of ED after fitting, the dashed line represents the significance association threshold. B: SNP-index correlation analysis results, the abscissa for chromosomal location, the black line for fitting after ΔSNP-index value, the red line represents the confidence level of 0.99 the threshold line, blue line represents the confidence level of 0.95 the threshold line, green line represents the confidence level of the threshold line of 0.90. The results of the two association analysis methods show that the correlation regions associated with the multifoliolate leaf trait is located at the end of chromosome 11.