甘蓝自交不亲和性相关基因BoGSTL21的克隆与表达分析
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左同鸿, 张贺翠, 刘倩莹, 廉小平, 谢琴琴, 胡燈科, 张以忠, 王玉奎, 白晓璟, 朱利泉
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Molecular cloning and expression analysis of BoGSTL21 in self-incompatibility Brasscia oleracea
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Tong-Hong ZUO, He-Cui ZHANG, Qian-Ying LIU, Xiao-Ping LIAN, Qin-Qin XIE, Deng-Ke HU, Yi-Zhong ZHANG, Yu-Kui WANG, Xiao-Jing BAI, Li-Quan ZHU
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表4 质粒共转化酵母的相互作用分析
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Table 4 Interaction analysis of plasmid co-transformation yeast
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编号
No. | 酵母菌种(质粒)
Yeast strain (plasmid) | 培养基
Yeast medium | 菌斑
Colony | 颜色
Color | | 1 | Y2HGold (pGADT7-T×pGBKT7-53) | SD/-Leu/-Trp | 是Yes | 白色White | | 2 | Y2HGold (pGADT7-T×pGBKT7-Lam) | SD/-Leu/-Trp | 是Yes | 红色Red | | 3 | Y2HGold (pGADT7-BoFAB1C×pGBKT7-BoGSTL21) | SD/-Leu/-Trp | 是Yes | 白色White | | 4 | Y2HGold (pGADT7-BoPATL2×pGBKT7-BoGSTL21) | SD/-Leu/-Trp | 是Yes | 白色White | | 5 | Y2HGold (pGADT7-BoF9N12×pGBKT7-BoGSTL21) | SD/-Leu/-Trp | 是Yes | 白色White | | 6 | Y2HGold (pGADT7-T×pGBKT7-53) | SD/-Ade/-His/-Leu/-Trp | 是Yes | 白色White | | 7 | Y2HGold (pGADT7-T×pGBKT7-Lam) | SD/-Ade/-His/-Leu/-Trp | 是Yes | 无色No | | 8 | Y2HGold (pGADT7-BoFAB1C×pGBKT7-BoGSTL21) | SD/-Ade/-His/-Leu/-Trp | 是Yes | 白色White | | 9 | Y2HGold (pGADT7-BoPATL2×pGBKT7-BoGSTL21) | SD/-Ade/-His/-Leu/-Trp | 是Yes | 白色White | | 10 | Y2HGold (pGADT7-BoF9N12×pGBKT7-BoGSTL21) | SD/-Ade/-His/-Leu/-Trp | 是Yes | 白色White |
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