甘蓝自交不亲和性相关基因BoGSTL21的克隆与表达分析

左同鸿, 张贺翠, 刘倩莹, 廉小平, 谢琴琴, 胡燈科, 张以忠, 王玉奎, 白晓璟, 朱利泉

Molecular cloning and expression analysis of BoGSTL21 in self-incompatibility Brasscia oleracea

Tong-Hong ZUO, He-Cui ZHANG, Qian-Ying LIU, Xiao-Ping LIAN, Qin-Qin XIE, Deng-Ke HU, Yi-Zhong ZHANG, Yu-Kui WANG, Xiao-Jing BAI, Li-Quan ZHU

图10 BoGSTL21蛋白的原核表达 M: 蛋白分子量标准; 1: BoGSTL21-pGEX-4T-1未诱导蛋白; 2: BoGSTL21-pGEX-4T-1诱导蛋白; 3: pGEX-4T-1蛋白; 4: BoGSTL21-pGEX-4T-1纯化蛋白。

Fig. 10 Prokaryotic expression of BoGSTL21 protein M: molecular weight standard of protein; 1: BoGSTL21-pGEX- 4T-1 is an uninduced control; 2: BoGSTL21-pGEX-4T-1 is the purified fusion protein; 3: pGEX-4T-1 protein; 4: BoGSTL21- pGEX-4T-1 purified protein.