甘蓝自交不亲和性相关基因BoGSTL21的克隆与表达分析

左同鸿, 张贺翠, 刘倩莹, 廉小平, 谢琴琴, 胡燈科, 张以忠, 王玉奎, 白晓璟, 朱利泉

Molecular cloning and expression analysis of BoGSTL21 in self-incompatibility Brasscia oleracea

Tong-Hong ZUO, He-Cui ZHANG, Qian-Ying LIU, Xiao-Ping LIAN, Qin-Qin XIE, Deng-Ke HU, Yi-Zhong ZHANG, Yu-Kui WANG, Xiao-Jing BAI, Li-Quan ZHU

图5 甘蓝BoGSTL21与其他物种的同源蛋白氨基酸序列比对 黑色: 氨基酸一致性为100%; 深灰色: 氨基酸一致性为75%; 白色: 氨基酸一致性为50%。三角形标记BoGSTL21和BoGSTL2的不同之处; 下画直线为GST-N端结构域, 下画虚线为GST-C端结构域, 同时也代表该蛋白在这一区段保守性高。

Fig. 5 Alignment of BoGSTL21 of Brassica oleracea with homologous proteins of other species Black: amino acid identity 100%; Dark gray: amino acid identity 75%; White: amino acid identity 50%. The triangle marks the difference between BoGSTL21 and BoGSTL2; the underlined line denotes the GST-N terminal domain, and the underlined line denotes the GST-C terminal domain, which also indicates that the protein is highly conserved in this segment.