甘薯IbCAF1基因的克隆及耐盐性、抗旱性鉴定

陈杉彬, 孙思凡, 聂楠, 杜冰, 何绍贞, 刘庆昌, 翟红

Cloning of IbCAF1 and identification on tolerance to salt and drought stress in sweetpotato

Shan-Bin CHEN, Si-Fan SUN, Nan NIE, Bing DU, Shao-Zhen HE, Qing-Chang LIU, Hong ZHAI

表1 本研究所用引物

Table 1 Primers used in this study

引物名称 Primer name 引物序列 Primer sequence (5°-3°) IbCAF1扩增引物 Primers for IbCAF1 amplification IbCAF1-F: ATGGGTGTACAAGAAGATGTTTTG IbCAF1-R: CTAAAAAACTTCTAGTCCGTACAATACT 鉴定转基因引物 Primers for identifying transformants IbCAF1-OPF: GAGGCTTACGCAGCAGGTC IbCAF1-OPR: TTATAATTCATACTCGTGGAAGCGC 实时定量PCR引物 Primers for real-time quantitative PCR IbActin-F: AGCAGCATGAAGATTAAGGTTGTAGCAC IbActin-R: TGGAAAATTAGAAGCACTTCCTGTGAAC Actin-F: GAGGAATGCAGATCTTCGTG Actin-R: TCCTTGTCCTGGATCTTAGC IbCAF1RT-F: TCAGCTACCTCATCGACGAC IbCAF1RT-R: AGTCAACCCGAGCTGAATCA SOD-F: CTATTACCGACAAGCAGATTCCTC SOD-R: TACCACAAGCAACCCTTCCAC APX-F: GATGTTCCCTTTCACCCTGG APX-R: CAGATAGACCCATTTGCTTCACA POD-F: TCCGGGAGCCACACCATTGG POD-R: TGGTCGGAATTCAACAG P5CS-F: TTGTGACACGGACTGATGGAA P5CS-R: TATCTAAGCCGCTGACGACCA