甘薯IbCAF1基因的克隆及耐盐性、抗旱性鉴定

陈杉彬, 孙思凡, 聂楠, 杜冰, 何绍贞, 刘庆昌, 翟红

Cloning of IbCAF1 and identification on tolerance to salt and drought stress in sweetpotato

Shan-Bin CHEN, Si-Fan SUN, Nan NIE, Bing DU, Shao-Zhen HE, Qing-Chang LIU, Hong ZHAI

图4 IbCAF1增强了转基因烟草植株的耐盐性和抗旱性 A: 转IbCAF1基因烟草植株和WT烟草植株在无胁迫或添加200 mmol L-1 NaCl或10% PEG-6000的1/2 MS培养基上培养4周; B~H: 在无胁迫、200 mmol L-1 NaCl或10% PEG-6000的1/2 MS培养基上培养4周的IbCAF1转基因烟草和WT烟草叶片的DAB染色(B)、NBT染色(C)、H2O2含量(D)、SOD活性(E)、POD活性(F)、脯氨酸含量(G)、丙二醛含量(H)。*与**分别表示在0.05和0.01水平下差异显著。

Fig. 4 IbCAF1 enhances salt and drought tolerance in transgenic tobacco plants A: responses of IbCAF1-transgenic and WT tobacco plants cultured for 4 weeks on half-MS medium supplemented without stress or with 200 mmol L-1 NaCl or 10% PEG-6000; B-H: DAB staining (B), NBT staining (C), H2O2 content (D), SOD activity (E), POD activity (F), proline content (G), and MDA content (H) in the leaves of IbCAF1 transgenic and WT tobacco plants cultured for 4w on half-MS medium supplemented with no stress, 200 mmol L-1 NaCl or 10% PEG-6000. * and ** indicate significantly different at the 0.05 and 0.01 probability levels, respectively.