小麦主栽品种济麦22与良星99的基因组序列多态性比较分析

杨正钊, 王梓豪, 胡兆荣, 辛明明, 姚颖垠, 彭惠茹, 尤明山, 宿振起, 郭伟龙

Comparative analysis of the genomic sequences between commercial wheat varieties Jimai 22 and Liangxing 99

YANG Zheng-Zhao, WANG Zi-Hao, HU Zhao-Rong, XIN Ming-Ming, YAO Ying-Yin, PENG Hui-Ru, YOU Ming-Shan, SU Zhen-Qi, GUO Wei-Long

表1 本研究使用的引物编号和序列

Table 1 Primer IDs and sequences used in this study

引物编号 Primer ID 引物序列 Primer sequence (5'-3') 退火温度 Annealing temperature (℃) 用途 Purpose TraesCS2D02G055700_F CAGGTCGAGACAGAGAACAA 56 测序引物 Sequencing primers TraesCS2D02G055700_R ATCGAGCCCCTCAATTTCAT 58 CNV标记特异引物 Specific primers for CNV markers TraesCS2D02G051500_F TCAGCTCAGGGTTATCAAGC TraesCS2D02G051500_R TTGGGTGCATTTTTCAGTCC 2B_LX99_CNV1_F AGCAGGTAATCCACACCAAA 2B_LX99_CNV1_R TGGAGAACCCACTTATCCAA 2B_LX99_CNV2_F AGCAGGTAATCCACACCAAA 2B_LX99_CNV2_R AATCCACACCAAATCCCCAT 2B_COM_CNV_F CACCCTAGATACAACCGAGG 2B_COM_CNV_R ATGTCACCGATCTTCTGAGC 2D_COM_CNV_F ATGATCACGATGGACCTAGC 2D_COM_CNV_R TGCCACGAAGATTTAGAGGA