红麻海藻糖生物合成关键酶基因HcTPPJ的克隆及响应逆境的表达分析

李辉, 李德芳, 邓勇, 潘根, 陈安国, 赵立宁, 唐慧娟

Cloning of the key enzyme gene HcTPPJ in trehalose biosynthesis of kenaf and its expression in response to abiotic stress in kenaf

Hui LI, De-Fang LI, Yong DENG, Gen PAN, An-Guo CHEN, Li-Ning ZHAO, Hui-Juan TANG

图2 红麻HcTPPJ与其他植物TPPJ蛋白氨基酸序列一致性比对 OsTPP1: 水稻; AtTPPA、AtTPPB、AtTPPD: 拟南芥; TPPJ: 可可树、木槿、榴莲、雷蒙德氏棉、毛果杨、红麻、橡胶树、石榴。不同颜色代表不同氨基酸残基的保守性。蓝色表示氨基酸完全保守; 粉红色、青色、黄色分别表示氨基酸的保守性为75%以上、50%以上及33%以上; 白色表示氨基酸的保守性不足33%。

Fig. 2 Amino acid sequence alignment of HcTPPJ and TPPJ proteins from other plants OsTPP1: Oryza sativa; AtTPPA, AtTPPB, AtTPPD: Arabidopsis thaliana; TPPJ: Theobroma cacao, Hibiscus syriacus, Durio zibethinus, Gossypium raimondii, Populus trichocarpa, Hibiscus cannabinus, Hevea brasiliensis, Punica granatum. The different colors indicate that conservatism of different amino acid residues. The blue means amino acids are complete conservation, the pink, cyan, yellow mean that the conservation of amino acids is no less than 75%, no less than 50%, no less than 33%, respectively, and white mean no more than 33%.