玉米雄性不育突变体mi-ms-3的遗传分析及分子鉴定
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田士可, 秦心儿, 张文亮, 董雪, 代明球, 岳兵
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Genetic analysis and characterization of male sterile mutant mi-ms-3 in maize
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TIAN Shi-Ke, QIN Xin-Er, ZHANG Wen-Liang, DONG Xue, DAI Ming-Qiu, YUE Bing
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表1 基因定位及基因表达分析的引物
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Table 1 Primers for genetic mapping and gene expression analysis
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引物名称 Primer name | 正向引物 Forward sequence (5′-3′) | 反向引物 Reverse sequence (5′-3′) | 染色体位置 Chromosome position | umc1973 | CAGGCAGAAAAGGAACGGAAC | GTGCGAGAGAAGATGGATGATTG | 3.05 | umc1027 | AACTCTGTCTCCGTCACCGTGT | GACCTCATCTCGGTGGAAATTG | 3.06 | S-1 | CCGTCATCGACATTTTACTCAG | CACAAGAGTTGGTTCGCTG | 3.06 | S-2 | CCTTGTACCCGCTCATCCAT | CTCCTACCGACCTGCCTG | 3.06 | S-3 | GTGTTACAAGAACTCAGCGT | AGCCTTCGTTCAGTTTCGGT | 3.06 | S-4 | TGACTGGCTTTCGTCCTACT | CACTCTCCAGCTGCTTCTCT | 3.06 | S-5 | ACAATTAGCTTAAGAACGC | AGCGGTGTTCATGGTACTCA | 3.06 | S-6 | TGGCTACTAGCTAGCTGTGCT | CTAAACCTTCACGCACAGCC | 3.06 | S-7 | CCAACTGAGCCGAATCGTTC | ATCATCCCATATCCCACGCC | 3.06 | S-8 | GTGGCCCATATGGTCTCAAC | CGGCCAGTCGATTAAAGCTC | 3.06 | S-9 | CGACGAAGAACTGACTTGACC | TTGCTTGATCATTCCTCCGC | 3.06 | S-10 | GTTTGGGCCGCATTCGAGAGA | TATTCTTGCCTCTCCCACCG | 3.06 | TIR-1 | CGGCCTCCATTTCGTCGAATCCCTT | | | 42618 | TGCCGGTACTGTGACTGTTC | CCTGAGCTCGATCTGCATGG | 3.06 | 42618-2 | TCTTTACTCCTCCCCTCCCA | CTTCTTGAGGATCCCGTTGC | 3.06 | q42618 | GCCTTAGTGCAGAGATTGACC | GCCTTTCCCAGTGCTCCA | 3.06 |
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