玉米ZmbHLH161基因的克隆及功能研究

杨梦婷, 张春, 王作平, 邹华文, 吴忠义

Cloning and functional analysis of ZmbHLH161 gene in maize

Meng-Ting YANG, Chun ZHANG, Zuo-Ping WANG, Hua-Wen ZOU, Zhong-Yi WU

图4 ZmbHLH161蛋白在玉米叶片原生质体中的亚细胞定位 GFP: GFP标记的ZmbHLH161在玉米原生质体的亚细胞定位; DAPI: DAPI标记的原生质体细胞核; Bright: 同一视野光镜下的原生质体; Merged: 光镜及荧光照片的叠加; CK: 转入空载体的原生质体; ZmbHLH161: 转入目的基因载体的原生质体; 标尺为3 μm。

Fig. 4 Subcellular localization of ZmbHLH161 protein in maize leaf protoplasts GFP: GFP-labeled ZmbHLH161 subcellular localization in maize protoplasts; DAPI: DAPI-labeled protoplast nuclei; Bright: protoplasts under the same field of view light microscope; Merged: superposition of light microscope and fluorescence photos; CK: transfer empty vector protoplasts; ZmbHLH161: transfer into protoplast of target vector; Bar = 3 μm.