甘蔗热带种金属硫蛋白家族基因的克隆及响应重金属胁迫的表达分析

高世武,傅志伟,陈云,林兆里,许莉萍,郭晋隆

Cloning and expression analysis of metallothionein family genes in response to heavy metal stress in sugarcane (Saccharum officinarum L.)

Shi-Wu GAO,Zhi-Wei FU,Yun CHEN,Zhao-Li LIN,Li-Ping XU,Jin-Long GUO

表1 甘蔗金属硫蛋白基因的克隆引物

Table 1 Primers for isolation of sugarcane metallothionein genes

基因 Gene 上游引物 Forward primer (5°-3°) 下游引物 Reverse primer (5°-3°) ScMT1 TGTAACACGCCCTTTGGTAGAT TGTCCTTATTGTTCAACCACCAG ScMT2-1-5 CGAGGAGAGGAAGAGGACGACT TTATTCGTTCCCATCAGTACCA ScMT3 AAGAAGGCCTTGCGCAACAAA ACGTACCGCGTCAGGTCCTCGT