小麦抗麦红吸浆虫基因标记的开发与验证

郝志明,耿妙苗,温树敏,闫桂军,王睿辉,刘桂茹

Development and validation of markers linked to genes resistant to Sitodiplosis mosellana in wheat

Zhi-Ming HAO,Miao-Miao GENG,Shu-Min WEN,Gui-Jun YAN,Rui-Hui WANG,Gui-Ru LIU

图2 EST标记E1-2 (A)和E10-10 (B)在抗、感虫小麦亲本及部分RIL系中的扩增结果 M: 分子量marker (pBR322/Mst I酶切片段); 1: 感虫小麦亲本6218; 2: 抗虫小麦亲本冀麦24; 3~14: 感虫株系(RIL-16、RIL-19、RIL-20、RIL-21、RIL-25、RIL-29、RIL-45、RIL-102、RIL-113、RIL-119、RIL-125和RIL-139); 15~26: 抗虫株系(RIL-7、RIL-12、RIL-18、RIL-23、RIL-64、RIL-73、RIL-91、RIL-115、RIL-134、RIL-156、RIL-169和RIL-170)。

Fig. 2 Polyacrylamide gel electrophoresis profiles for EST markers E1-2 (A) and E10-10 (B) in susceptible, or resistant-wheat parent, and selected RIL lines M: Molecular marker (restriction fragments of plasmid pBR322 digested with Mst I endonuclease); 1: Susceptible wheat parent ‘6218’; 2: Resistant parent ‘Jimai 24’; 3-14: Susceptible lines (RIL-16, RIL-19, RIL-20, RIL-21, RIL-25, RIL-29, RIL-45, RIL-102, RIL-113, RIL-119, RIL-125, and RIL-139); 15-26: Resistant lines (RIL-7, RIL-12, RIL-18, RIL-23, RIL-64, RIL-73, RIL-91, RIL-115, RIL-134, RIL-156, RIL-169, and RIL-170).