诸葛菜小孢子培养及其单倍体减数分裂染色体配对观察

殷家明,钟荣棋,林呐,唐章林,李加纳

Microspore culture and observations on meiotic chromosome pairing of the haploid in Orychophragmus violaceus

Jia-Ming YIN,Rong-Qi ZHONG,Na LIN,Zhang-Lin TANG,Jia-Na LI

图1 诸葛菜小孢子胚胎发生过程 A: 部分小孢子膨大; B: 小孢子近均等分裂; C: 小孢子不均等分裂; D: 多细胞团; E: 具胚柄状结构的原胚; F: 球形原胚; G: 球形胚; H: 具胚柄状结构的圆球形胚状体; I: 卵球形胚状体; J: 桃心形胚状体; K: 心脏形胚状体; L: 培养28 d后的胚状体。

Fig. 1 Microspore embryogenesis of Orychophragmus violaceus A: some swelled microspores; B: microsporal equal cell division; C: microsporal unequal cell division; D: multicell cluster; E: proembryo with a suspensor-like structure; F: globular proembryo; G: globular embryo; H: round sphere embryoid with a suspensor-like structure; I: oval sphere embryoid; J: heart embryoid; K: cordate embryoid; L: embryoids after 28 days of culture.