诸葛菜小孢子培养及其单倍体减数分裂染色体配对观察

殷家明,钟荣棋,林呐,唐章林,李加纳

Microspore culture and observations on meiotic chromosome pairing of the haploid in Orychophragmus violaceus

Jia-Ming YIN,Rong-Qi ZHONG,Na LIN,Zhang-Lin TANG,Jia-Na LI

图3 诸葛菜小孢子植株的再生、形态、育性和倍性 A: 胚状体萌发; B: 加倍的小孢子植株; C: 单倍体植株; D: 加倍和单倍体植株的花和花蕾; E: 加倍植株花粉; F: 单倍体植株花粉; G: 加倍植株花粉母细胞染色体(2n = 24); H: 单倍体植株花粉母细胞染色体(2n = 12); 标尺 = 10 μm。

Fig. 3 Regeneration, morphology, fertility, and ploidy of the microspore-derived plantlets in O. violaceus A: germination of embryoids; B: doubled microspore-derived plantlet; C: haploid plantlet; D: flowers and buds of double haploid and haploid plantlets; E: pollens of double haploid plantlet; F: pollens of haploid plantlet; G: chromosomes in the pollen mother cell of doubled plantlet (2n = 24); H: chromosomes in the pollen mother cell of haploid plantlet(2n = 12); bar = 10 μm.