基于QTL定位和全基因组关联分析筛选甘蓝型油菜株高和一次有效分枝高度的候选基因

霍强,杨鸿,陈志友,荐红举,曲存民,卢坤,李加纳

Candidate genes screening for plant height and the first branch height based on QTL mapping and genome-wide association study in rapessed (Brassica napus L.)

Qiang HUO,Hong YANG,Zhi-You CHEN,Hong-Ju JIAN,Cun-Min QU,Kun LU,Jia-Na LI

表1 候选基因引物及其扩增产物

Table 1 List of primer sequences for candidate genes

基因名 Gene name 引物序列 Primer sequence (5°-3°) BnaA03g47940D F: ATGGAGGCAATGAAGATGAAAC; R: GATAGACAAAGAAGCATCAGAG BnaA07g28720D F: CAGCTTCTTCGCTTACAAGATC; R: ATGCTCGATGGCTTATACTCAA BnaA07g28820D F: TTTTACTCGTCGTGCTTCTCTC; R: GACCTTCGAAAAACATGATGCT BnaC06g30400D F: ACAAGAATAATCGTTCTGCTGC; R: CCAACAGTTTCACTCTTCAAGG BnaC06g30410D F: TGGAAGCGTTACTGATTTGATG; R: TGAATATTGAGTGCAAGTAAGC BnaA01g29630D F: ATAAAGACTCTATCGCGATCGG; R: ATCCAAGGAGCGATATTAGTGG BnaA01g30830D F: ACTCTTTCGGTTCTTTTGTTGG; R: GTTTTGTTACGTCCGAAGAACA BnaA02g31980D F: GACGATGTCAAGCTTATAAGCG; R: TAACCCCTAAACAAACCTCTCC BnaA02g37010D F: TTCTTGAATTGGCAGAACGATC; R: CTTCCGTGACAACAACCTTTAG BnaA09g48360D F: CAGTCAAAGCCATCCATGAAAA; R: CGAAGCTAATAGTGTGTTGGTG BnaA09g49220D F: TTGCTTCTTTTGTCAACCTAGC; R: CAGAAACAGTGCATACAGCTAC BnaC02g14570D F: GTGTTCTCAGGGAGATATCTCG; R: CACGAGGATCTTCAAATCCAAC