利用EST-SSR标记评价羽扇豆属(Lupinus L.)遗传多样性

张红岩,杨涛,刘荣,晋芳,张力科,于海天,胡锦国,杨峰,王栋,何玉华,宗绪晓

Assessment of genetic diversity by using EST-SSR markers in Lupinus

Hong-Yan ZHANG,Tao YANG,Rong LIU,Fang JIN,Li-Ke ZHANG,Hai-Tian YU,Jin-Guo HU,Feng YANG,Dong WANG,Yu-Hua HE,Xu-Xiao ZONG

附表 2 95对多态性EST-SSR信息

Supplement table 2 Characteristics of 95 pairs of polymorphic primers

引物 Primer 重复单元 Repeat motif 正向引物 Forward sequence (5°-3°) 正向引物 Reverse sequence (5°-3°) 退火温度 Tm (℃) lup-est6104 (TTG)4 CTCAAGTTTTGGCTTCATGC CTCAAGTGCATTCATGGAAGT 54 lup-est4304 (TC)6 AGGGGTTTTGAAGAGAAAGG AGACAGTGATAGCTCAGTGGG 54 lup-est2704 (GA)5 TCAAGCCCTTCACTCAAAAC CCCCCTTTCTAAAAATCCAA 54 lup-est2313 (TC)4 GTGACAATGGCTCTTCAACC CCTTGTGCAAAAGTCCAAAT 54 lup-est713 (TAA)4 CAGCCATTAAAGCTTTTGGA AGAAAACACCAAAAGGGACC 54 lup-est613 (TTG)5 TTCCACCCATCATTGAAATC GGAGCAACAACTACACATCCA 54 lup-est1313 (TTA)5 GAAAACCACCACCACTTTTG GGGGGTAGTGCTAAGAAAGG 54 lup-est4458 (CAA)3 CAGAATCACACCCCAATCTC ATCCCAGGCCATTGACTAAT 54 lup-est5958 (AAC)4 CAACGGGGTCATTGAATTAG GTTTGTGACGGGTTAAGGTG 54 lup-est3458 (CAC)4 GACGACTCCAATGACACTCC GATGAGGGTTTCGAAGAGGT 54 lup-est1058 (GGT)4 TTTATCGGGAAGCATGTGTT ATAGGTGAGCCTGGGACTTC 54 lup-est2558 (CT)9 AGACCCACCAAACAATGAAA ATCCCGAGGCCAAATCTA 54 lup-est158 (CTT)3 TGGTTGTTGGGTCTAATAGCA TGAGGAGAACCATCATTCAAG 54 lup-est6558 (ATG)6 TGTTGATGCCAGAAACATTG GCCTTCCCTTCTCTACAACC 54 lup-est4949 (TGA)5 CTCAATCCCCTAGTGACCCT GGTAATTCCAAACTTGCCCT 54 lup-est1649 (TC)6 CAATCTTCTTCACTCTACTCCTCA CAAGTCTGAAATCCTTGCGT 54 lup-est1449 (TTG)6 GGTTTCCATCATAAGCATCC TCAGCACACTAGCAAGTACCC 54 lup-est5740 (AAT)5 ACATGTGCATGTTGAATTGC ATTTCGAGGACCAACAATGA 54 lup-est2540 (CAC)4 GAATCATTGAACCGTGATGG GAAAGGGTTCTCTGCTTCGT 54 lup-est4549 (TTG)4 AACCCCATTTTAAGCAAGGT CCACTCTTCCTCAGAAAAATCA 54 lup-est2949 (CAA)5 ATCAATGGCACAAGCAATG AACCAGTAGCAACAAGACCAA 54 lup-est3140 (CTT)4 CATTCAACCAAAACCCATGT ACACGCAGTTTGAAGAGAGC 54 lup-est2849 (TTC)5 CGATGGTCCATTGATATAGAAG AGACCTTCCGAAGTGAGACA 54 lup-est1149 (TGT)4 GCAATGTTGAGCTTCCAAAG CTTTCTTGGTGTTGGAGGAA 54 lup-est4640 (TTG)7 CCTTACCATTGCATCAGTCC GGTATAACCCCTTTTCAGCC 54 lup-est6031 (AAC)4 AAATCTAAGCCCATGGAACC CCCTGCTCTTCTCAAAGTCA 54 lup-est840 (ATC)5 CAACTTTTCAACGGGTTCAC GCAGAAGGGTAAAATACGCA 54 lup-est931 (GAA)4 CAATTTCACCCTCTTCGCTA CTTTAGCAACATCGAAGGGA 54 lup-est6531 (CCT)4 CAAGGTTTGCTTCGTAGGAG AGGGAGGAAATTGGATGTGT 54 lup-est1985 (TGC)4 AAGTGATGTCCTGAGCTTGC GCTCCACAGATGACACAACA 54 lup-est4185 (GTG)5 GCAACAGTCACCTTGCATTA CCCTTAAACTCCACTTCAACC 54 lup-est3185 (CAA)5 CTCAACCCAACAAAACAACA GCACTGCAATCAGAAGTGAA 54 lup-est6567 (CTA)5 CCAACTCCAGTCACTGCTCT GCTCTCAAATGGGGTTGTAG 54 lup-est2567 (TAG)6 AGTGGCCAGGATAGTGTTGA CAAACTCCACCTCATTGTCC 54 lup-est5667 (GGA)4 CAAGGGTAAGTACCCGATCC GAGAGATGAGTGAAGCACAGAA 54 lup-est2076 (GGT)3 ATAGTTGCTGTAACTCGGCG CTGTCCTCCTCCTCCTTCTC 54 lup-est4467 (ACA)4 TGCATTCATGGCTTCACTTA TTGTGACAACTTGGAGGGAT 54 lup-est976 (GAA)4 GCTTAAGGGAGGAGCAAGAT GCTTCTTGTGCTGCTCTTTC 54 lup-est1622 (CAC)4 GTACCCCTGATGATGCTGAC TGGTGGATTGTCTGATGATG 54 lup-est4022 (GAA)4 TCTACTTCCTTCACAAAACGC CAAAAGGTAACGAAGCTCCA 54 lup-est822 (ATA)4 CATATTCAAGGCTTTGGTGG TTTGGGGCAAGTAGAAATTG 54 lup-est4522 (ATG)5 AGGTAACGTTGATTCGTGGA CACCAACAACACCACTACCA 54 lup-est2822 (CCT)4 AGACCAAGAGAGTGGAGGCT CACTCTCTCAGTAGGAGGCG 54 lup-est6213 (AAG)5 GGCGTGGGTTAAGCTTATGT ACCCCACCTTCTTCAATCAT 54 lup-est2694 (ATC)6 GGGGCTGCTTCATCTTATTT CGCCTCAATTCAAGGATATG 54 lup-est1594 (ATC)6 GAATGTTCCAGTTGGTGGAG ATCATAACCCATTGACCCCT 54 lup-est3894 (TC)6 GAAACCCAACCAATTGTTCA AACCAACATTCTCCACTTGC 54 lup-est2013 (ATG)4 CTATGGAAGATGCGCAGAAT CCCACTGAATCTGACTTGCT 54 lup-est6504 (ACC)4 TGCCTTCTATTGTCACATGC AGATTCTGACTAGCCCCACC 54 lup-est2404 (TGG)4 AAGCACCACCTTCTGTGCTA TCACTTTGGTTCCACTAGCC 54 lup-est6158 (AAC)4 ACCAAAGAAAACAAAACCCC GGACGTCCTTGTGCTGTTAC 54 lup-est458 (CGC)4 AGAGAAGAAACCGGAGGAAA GCATGAACACTTTCAGCACA 54 lup-est4158 (GTT)4 AGAGAGACCAGTGGGAGGAT AAAACCTGTTCGGCTTCTTT 54 lup-est6049 (TGG)5 CTGGCAGCATGGTTTTTAAG AAATGGACTTGTCCCAAACC 54 lup-est58 (GA)7 TCCAGCAGATCCGTTAATCT TCAGATCGCACTCTCTGTGA 54 lup-est2358 (TTG)4 GGTTGATGCAGCAGAAAAGT ACAACTTGCAAATCCACCAT 54 lup-est5049 (AAC)4 TCAGTTAAATCCAACGCCAC GGAATTCTGCGAAGGTATCA 54 lup-est5749 (TGC)4 TGTCTCGATGGTTCAGCATA GCCTTAGATGCCCATCTTCT 54 lup-est6258 (AAT)5 GCTGAAATTGTAGCAGCCAT TGGACCTTGTTTTTCAATGC 54 lup-est3340 (ATG)7 AAGGTTCAGGACTTGTTGAAGA ATCAACCTGTGACCCAGAAA 54 lup-est449 (CAG)6 AGCTCCTCCCAATGCTTATT ATGTTCCAGAGAAATGCCAG 54 lup-est3649 (ACA)4 ACTCAAGTCCAACAATCCCA CACCTTAAACGTGAGAAAGCA 54 lup-est2340 (CTA)5 AAACCCAGCAAATTCACAAG TCTGGAACTCCATTCTTTGC 54 lup-est5440 (TAT)4 GGCTTTTCTTCCCATTGTTT TTTCAGAACAACGTGTCCAA 54 lup-est4349 (AAC)5 AAGAAATGCCCTAGCTGGTT TCTTGTTGCTCTTCCTTTGG 54 lup-est3549 (CT)6 CATGTGCACTCCGTTTATCA GACCTTGTTGTGGGTATTGC 54 lup-est249 (ATG)4 ACAGGTCCATGGAAAACAGA GGTGATGAATTGGGATCTGA 54 lup-est5340 (CCT)5 CGAGAAGCGATCGACATAAT CGACACAGCAGAAGTTGTGA 54 lup-est4249 (CTT)6 AGCTTTGATTTTTGACTGCG TCGTAGAAGTGGATACCCCA 54 lup-est2140 (CAA)4 AGGAACAATCTCAAGCCACA GAGATTATGCTGCCACTGCT 54 lup-est1749 (TGC)3 GCGTAGCTGCTCTCATTTTC AGCTCCACATTCATCCTCAG 54 lup-est5240 (AAC)3 GCAAACTCATTCCCTTCAAA CCTCATCAACGACACCTTCT 54 lup-est1640 (TTC)4 TTAGGGCATTGAACCTTTTG CCACCGTCGTAAGTAACACC 54 lup-est5831 (CTT)5 CACAGACTCAACCTTGGCTT TACTGGCTCCAGATCAAACC 54 lup-est4131 (GTT)6 CAAGCCCCTTAACAAGTCCT CTGGCCAGAGCTGGTAAATA 54 lup-est2531 (TC)6 CACGTTGGGTTTCTTTCATT GAGAGCTTTGCACGTTGATT 54 lup-est2185 (CCA)4 CACTCTTCCAAACGCAAGTT AGCTTCTTTAGGGTTCGGAG 54 lup-est5985 (GAA)5 CCCCAATCTCAGAAATCAAA CAAGTCAGCTTGGGAAAGAA 54 lup-est4576 (ATT)6 CCATATAGCTGCTTCCTCCA AATGCACTTCTCCACCACTC 54 lup-est1085 (CCA)4 AACCTATCTGCTGCTGATGC AGTTCTGCATGCAATCTGGT 54 lup-est2385 (ATG)4 CATGAAGAAGAAGCCAAGGA TTCACCACTTTCATCTTCAGC 54 lup-est3067 (TCA)4 CAAAGATGCTCATCAAGAAGC TAGGGGTTGTTCTTTGTCCA 54 lup-est2267 (GTT)5 AGCCAAGTAGGTGCTGTTTG TCACCACCTCTCTCAAAACC 54 lup-est3576 (CAC)4 CTGGGAATTGAGGTTTGTTG ACTGAAGACGGTGCTGATTC 54 lup-est5967 (AAC)4 AAATCACAATCACCACAGCA TGCATTAGGGAGAAAGTTGG 54 lup-est922 (TTC)4 AGTAGGGTGGAGATGGAAGG GCTTCTCCATGCTCTCTTCA 54 lup-est5622 (ACC)5 TGCTCCACTTTGTTCTTTCC TTGGCTATGGCAGTTTGAAT 54 lup-est6513 (CAA)5 ACCCTTCACAGACATCTCCA TAAGAGACAACATTGGGGGA 54 lup-est4113 (TGG)5 GGTGTTGTTGGAGAGAAGGA TGGTTTTGTTGGCTGAGATT 54 lup-est5513 (ACT)4 AATTCACACGTGGAGGATTC GAGGATCCAAGTTTTGTGGA 54 lup-est4322 (TCT)4 CTGTGCTGGACTTGGTATCC CTTTGCATATTGGTTTGCCT 54 lup-est3494 (CTT)6 TAGCACTGGTTTTCATTGCC CCTGGTGAACCTGATTGAAG 54 lup-est3994 (ACT)4 GGAAACTCCACAAAACCCTT TGTGATACACTGGAATCCCC 54 lup-est2194 (AAT)6 GTGATCAAGATGTGGCAGTG GTTTGTTGTTGGTGCAATGA 54 lup-est164 (CAA)3 AACAAGATTTGGGAGGTTCC CCATCCGGAAGACATATTCA 54