高温胁迫终止后Bt棉蕾杀虫蛋白的恢复特征及相关生理机制

刘震宇,王桂霞,李丽楠,蔡泽洲,梁潘潘,吴莘玲,张祥,陈德华

Recovery characteristics of Bt insecticidal protein and relative physiological mechanisms after high temperature stress termination in square of Bt cotton

Zhen-Yu LIU,Gui-Xia WANG,Li-Nan LI,Ze-Zhou CAI,Pan-Pan LIANG,Xin-Ling WU,Xiang ZHANG,De-Hua CHEN

表1 高温持续胁迫终止后蕾中可溶性蛋白与游离氨基酸含量变化

Table 1 Changes of square soluble protein and free amino acid contents with time after high temperature stress termination

测定指标 Tested index	品种 Variety	处理 Treatment	恢复时间 Recovery time
测定指标 Tested index	品种 Variety	处理 Treatment	0 h	12 h	24 h	48 h	72 h	96 h
可溶性蛋白含量 Soluble protein content (mg g^-1FM)	SK-1	CK	10.19 a	10.25 a	10.25 a	10.19 a	10.16 a	10.26 a
		38℃-72 h	4.77 d	5.12 d	6.45 c	8.56 b	10.36 a	10.36 a
		38℃-96 h	4.15 e	4.44 e	5.43 d	6.86 c	8.47 b	10.09 a
	SK-3	CK	12.10 a	12.20 a	12.27 a	12.25 a	12.18 a	12.24 a
		38℃-72 h	6.26 d	7.42 c	9.17 b	12.05 a	12.23 a	12.27 a
		38℃-96 h	5.93 e	6.80 d	7.88 c	10.89 a	12.02 a	12.23 a
游离氨基酸含量 Free amino acid content (μmol g^-1FM)	SK-1	CK	255.21 a	250.16 a	249.88 a	251.92 a	253.55 a	249.60 a
		38℃-72 h	747.74 a	693.73 b	626.64 c	476.67 d	240.14 d	243.28 d
		38℃-96 h	974.31 a	923.16 b	854.25 c	689.28 d	500.86 e	252.38 e
	SK-3	CK	277.06 a	273.20 a	281.32 a	277.44 a	275.88 a	272.99 a
		38℃-72 h	681.54 a	607.52 b	520.30 c	286.08 d	279.63 d	272.75 d
		38℃-96 h	905.24 a	829.97 b	742.43 c	346.71 d	273.32 e	274.59 e