高温胁迫终止后Bt棉蕾杀虫蛋白的恢复特征及相关生理机制

刘震宇,王桂霞,李丽楠,蔡泽洲,梁潘潘,吴莘玲,张祥,陈德华

Recovery characteristics of Bt insecticidal protein and relative physiological mechanisms after high temperature stress termination in square of Bt cotton

Zhen-Yu LIU,Gui-Xia WANG,Li-Nan LI,Ze-Zhou CAI,Pan-Pan LIANG,Xin-Ling WU,Xiang ZHANG,De-Hua CHEN

表3 高温持续胁迫终止后蕾中蛋白酶与肽酶活性变化

Table 3 Changes of square protease and peptidase activities with time after high temperature stress termination

测定指标 Tested index 品种 Variety 处理 Treatment 恢复时间 Recovery time 0 h 12 h 24 h 48 h 72 h 96 h 蛋白酶活性 Protease activity (μg g-1 h-1) SK-1 CK 34.62 a 34.08 a 34.88 a 33.86 a 35.07 a 34.58 a 38℃-72 h 125.79 a 114.46 b 100.05 c 73.23 d 35.43 d 34.88 d 38℃-96 h 153.95 a 145.35 b 131.47 c 106.43 d 76.00 e 35.35 e SK-3 CK 39.84 a 40.76 a 40.98 a 40.67 a 39.74 a 40.19 a 38℃-72 h 114.88 a 99.46 b 83.75 c 39.08 d 41.16 d 39.94 d 38℃-96 h 141.91 a 122.30 b 105.78 c 62.09 d 43.01 e 41.49 e 肽酶活性 Peptidase activity (μmol g-1 h-1) SK-1 CK 1.28 a 1.28 a 1.29 a 1.27 a 1.28 a 1.27 a 38℃-72 h 3.43 a 3.24 a 3.06 ab 2.46 b 1.26 d 1.27 d 38℃-96 h 4.07 a 3.91 b 3.72 c 3.15 d 2.50 e 1.30 f SK-3 CK 1.64 a 1.61 a 1.62 a 1.62 a 1.61 a 1.60 a 38℃-72 h 3.20 a 2.77 b 2.32 c 1.56 d 1.62 d 1.59 d 38℃-96 h 3.79 a 3.48 b 3.11 c 2.07 d 1.59 e 1.59 e