转基因玉米MIR604基体标准物质研制

李俊,李亮,李夏莹,宋贵文,沈平,张丽,翟杉杉,柳方方,吴刚,张秀杰,武玉花

Development of genetically modified maize MIR604 matrix reference materials

Jun LI,Liang LI,Xia-Ying LI,Gui-Wen SONG,Ping SHEN,Li ZHANG,Shan-Shan ZHAI,Fang-Fang LIU,Gang WU,Xiu-Jie ZHANG,Yu-Hua WU

表1 引物探针信息表

Table 1 List of primer and probe information

靶标 Target 引物名称 Primer name 序列 Sequence (5°-3°) 产物大小 Amplicon size (bp) 参考文献 Reference MIR604 MIR604F GCGCACGCAATTCAACAG 76 Mazzara et al. 2007[10] MIR604R GGTCATAACGTGACTCCCTTAATTCT MIR604P AGGCGGGAAACGACAATCTGATCATG Adh1 ADH-FF3 CGTCGTTTCCCATCTCTTCCTCCT 135 Mazzara et al. 2013[10] ADH-RR4 CCACTCCGAGACCCTCAGTC ADH1-MDO AATCAGGGCTCATTTTCTCGCTCCTCA MON810 MON810F TCGAAGGACGAAGGACTCTAACGT 92 ISO 21570[11] MON810R GCCACCTTCCTTTTCCACTATCTT MON810P AACATCCTTTGCCATTGCCCAGC MON863 MON863F TGTTACGGCCTAAATGCTGAACT 84 Mazzara et al. 2005[12] MON863R GTAGGATCGGAAAGCTTGGTAC MON863P TGAACACCCATCCGAACAAGTAGGGTCA NK603 NK603-F ATGAATGACCTCGAGTAAGCTTGTTAA 108 Mazzara et al. 2005[13] NK603-R AAGAGATAACAGGATCCACTCAAACACT NK603-PR TGGTACCACGCGACACACTTCCACTC T25 MLD143 ACAAGCGTGTCGTGCTCCAC 102 Mazzara et al. 2013[14] MDB551 GACATGATACTCCTTCCACCG TM016 TCATTGAGTCGTTCCGCCATTGTCG TC1507 TC1507F TAGTCTTCGGCCAGAATGG 58 Mazzara et al. 2007[15] TC1507R CTTTGCCAAGATCAAGCG TC1507P TAACTCAAGGCCCTCACTCCG MIR604插入位点 MIR604 insert site MIR604GF GACGCCAGATCACACATG 168 本研究 This study MIR604GR CACACCTCGTTGCCAAAG MIR604GP CGTGCCGATCTCGCACCAGCA