玉米HD-ZIP I亚家族基因鉴定及表达分析

梁思维,姜昊梁,翟立红,万小荣,李小琴,蒋锋,孙伟

Genome-wide identification and expression analysis of HD-ZIP I subfamily genes in maize

Si-Wei LIANG,Hao-Liang JIANG,Li-Hong ZHAI,Xiao-Rong WAN,Xiao-Qin LI,Feng JIANG,Wei SUN

图3 玉米、水稻和拟南芥HD-ZIP I 亚家族蛋白的系统进化分析 所有HD-ZIP I亚家族基因的蛋白序列均来自于各自的基因组数据库: 玉米来自MaizeGDB (http://maizegdb.org/)、水稻来自TIGR (http://www.tigr.org/)和拟南芥来自TAIR (http://www.arabidopsis.org/)。利用ClustalX 2.0软件进行氨基酸序列的多重比对并用MEGA 5.1 软件构建Neighbor-Joining进化树(Bootstrap分析采用1000次重复)。黄色背景表示拟南芥基因, 绿色背景表示水稻基因, 紫色背景表示玉米基因。

Fig. 3 Phylogenetic tree of HD-ZIP I proteins in maize, rice, and Arabidopsis The HD-ZIP I subfamily protein sequences of maize, rice, and Arabidopsis were obtained from the MaizeGDB (http://maizegdb.org/), TIGR (http://www.tigr.org/), and TAIR (http://www.arabidopsis.org/) databases, respectively. Multiple alignment of amino acid sequences was carried out by ClustalX 2.0 software and Neighbor-Joining evolutionary tree was constructed by MEGA 5.1 (1000 replications of bootstrap test). Arabidopsis genes are highlighted in yellow background, rice genes are highlighted in green background, and maize genes are highlighted in purple background.