甘蔗栽培种单倍体基因组SSR位点的发掘与应用

王恒波,祁舒婷,陈姝琦,郭晋隆,阙友雄

Development and application of SSR loci in monoploid reference genome of sugarcane cultivar

Heng-Bo WANG,Shu-Ting QI,Shu-Qi CHEN,Jin-Long GUO,You-Xiong QUE

表4 5种禾本科植物中前3种最长SSR基序类型

Table 4 The first three longest SSR motifs in the five Gramineae plants

项目 Item 甘蔗 S. spp 高粱 S. bicolor 玉米 Z. mays 水稻 O. sativa 二穗短柄草 B. distachyon 单核苷酸 (T)75 (A)71 (A)88 (C)51 (A)49 Mono-nucleotide (T)63 (A)59 (A)85 (A)49 (C)45 (G)49 (A)53 (A)83 (A)48 (A)43 二核苷酸 (TA)71 (AT)280 (AC)1366 (AC)170 (AT)312 Di-nucleotide (TG)69 (AT)276 (AC)910 (AT)104 (AT)182 (TA)55 (AT)270 (AT)178 (AT)100 (AT)158 三核苷酸 (TGT)123 (ACT)366 (ACC)291 (AAT)165 (AAT)225 Tri-nucleotide (ATT)59 (AAT)327 (AAT)207 (AAT)147 (AAT)171 (TTA)56 (AAT)318 (ACT)132 (AAT)126 (AAT)138 四核苷酸 (TTAT)23 (ACAT)524 (ACAT)196 (ACAT)132 (ACAT)196 Tetra-nucleotide (ACAT)25 (AGAT)388 (ACAT)144 (ACAT)96 (ACAT)180 (ATGT)26 (ACAT)260 (AAAG)100 (ACAT)96 (ACAT)180 五核苷酸 (CTTTT)29 (AATAT)740 (AATAT)115 (AATAT)55 (AGATC)100 Penta-nucleotide (TTTTG)25 (AATAT)430 (ACTAT)115 (AATAT)55 (ACGCC)75 (AATAT)24 (AATAT)315 (AATAT)85 (AATAT)55 (AGATG)65 六核苷酸 (ATTGTC)43 (AAATAT)390 (AATAGT)198 (ACCTAT)90 (AACAGC)90 Hexa-nucleotide (TTTTTG)32 (AGATAT)366 (AATAGT)72 (ACATAT)78 (ACTGAT)78 (TTATAT)16 (AAATAT)294 (AACCAT)66 (ACATAT)78 (AGAGAT)66