甘蔗栽培种单倍体基因组SSR位点的发掘与应用
|
|
王恒波,祁舒婷,陈姝琦,郭晋隆,阙友雄
|
Development and application of SSR loci in monoploid reference genome of sugarcane cultivar
|
|
Heng-Bo WANG,Shu-Ting QI,Shu-Qi CHEN,Jin-Long GUO,You-Xiong QUE
|
|
表5 具有扩增多态性的甘蔗SSR引物信息表
|
Table 5 Primers information of sugarcane SSR with polymorphic amplification
|
|
引物名称
Primer name | 重复基序
Motif | 左引物序列
Left-primer (5°-3°) | 退火温度
Tm (℃) | 右引物序列
Right-primer (5°-3°) | 退火温度
Tm (℃) | 产物大小
Product size (bp) | PIC | | FAFUR-S1 | (TG)69 | TCATACCCATTGGAAGAAGC | 60.5 | GTTATGTTGCCGTGCCAAGT | 59.8 | 278 | 0.85 | | FAFUR-S3 | (TG)39 | TAGCCTTTGGTCGTTCTTGG | 58.2 | AATGCTTCATCCATAGGGGA | 59.3 | 259 | 0.84 | | FAFUR-S7 | (TG)32 | GCCTGGGGAACTATGCTGTA | 59.1 | CAAGCATTGAAGTTGCCAAA | 59.0 | 254 | 0.61 | | FAFUR-S12 | (TG)24 | CGTCAGTTGCTCAGCTCTTG | 58.0 | CCCTGGGAAGAAGAGGTAGG | 58.6 | 223 | 0.69 | | FAFUR-S17 | (TG)19 | AATGATGTTTCGCCTGATCC | 60.2 | ACCAACACAACTCGCTACCC | 60.1 | 166 | 0.77 | | FAFUR-S18 | (TG)19 | CCACATTCTTCGACCCTGTT | 59.8 | CCATCCTGCGAACTAACCAT | 59.7 | 183 | 0.71 | | FAFUR-S22 | (TG)18 | AGGGCACGAGGTATTGCTTA | 58.9 | AACCGGTCAAATCACACACA | 59.2 | 179 | 0.68 | | FAFUR-S24 | (TG)17 | ATCTTTCGGCATCAACTTGG | 60.1 | GCTTCAAGCCATCTGTCTCC | 60.3 | 274 | 0.73 | | FAFUR-S32 | (TG)13 | CAACGAATTCCACTTGCACA | 60.0 | TCATGGCTATTGTGGTCTGG | 60.4 | 207 | 0.61 | | FAFUR-S33 | (TG)13 | CTCCTCTGTCACCCAGCACT | 58.8 | GATCACCCCAGATCCAGAGA | 59.6 | 179 | 0.75 | | FAFUR-S34 | (TG)13 | TGCTGATTATGTGCTGCCTC | 58.5 | CACGCCTAGGGCATAAGAGA | 58.4 | 222 | 0.67 | | FAFUR-S36 | (TG)12 | AGGCATGGGAATTTCTCTCC | 60.1 | GGCCTCTCTTTAGTGCAGGA | 59.8 | 265 | 0.77 | | FAFUR-S38 | (TG)12 | GACACCCACCACAGGACTTT | 60.3 | CCCTCCCCAATCCTATCAGT | 60.1 | 198 | 0.66 | | FAFUR-S40 | (TG)11 | GCTGATGTTTGGTCATGTGG | 61.0 | TGCAGACTCAGAAGTAGCCG | 60.5 | 246 | 0.86 | | FAFUR-S41 | (TG)11 | TGTTTCAGGCACTGTTTTGG | 60.9 | AGCAATGTGTTCTCCATCCA | 60.5 | 261 | 0.73 | | FAFUR-S42 | (AG)38 | CGGCACAAGTAAATGCAAGA | 59.7 | AGTACTGCCAACAAGGCAGG | 58.3 | 230 | 0.85 | | FAFUR-S43 | (AG)34 | CTTGAGCTCGTAGCCTCCTC | 60.3 | GCCTCTGCTGTCTGCTCTCT | 59.6 | 267 | 0.92 | | FAFUR-S44 | (AG)31 | AGTGCAGGTTGGCTTTCTGT | 60.2 | GGGGATTCCAAGTCTCAACA | 59.8 | 206 | 0.82 | | FAFUR-S47 | (AG)25 | GTACCAGCCCAAAAACTGGA | 59.8 | TTGTCACTGGGAACACGGTA | 60.1 | 280 | 0.73 | | FAFUR-S49 | (AG)23 | TTCTCCGTCAACTGTCATGC | 59.6 | TCCTACGGAGGGAAATCAAA | 60.2 | 273 | 0.81 | | FAFUR-S4# | (TG)33 | CGACTGGAAGAAGATCGAGG | 58.2 | GAGGTACTGCATGCCCAAAT | 60.1 | 185 | - | | FAFUR-S5# | (TG)33 | CTTCCTCCCAGTAGCCGAGT | 59.3 | TCTCGAATTCGCAAGGAACT | 57.9 | 257 | - | | FAFUR-S6# | (TG)32 | GGAAGGAGGAGATGGAAAGG | 59.4 | CGCAACACGTACACACACAC | 59.6 | 245 | - | | FAFUR-S9# | (TG)26 | GTTTTCTTCTCGGAGGGGAG | 57.9 | AATGCTGGGATCGAAGTTTG | 60.2 | 213 | - | | FAFUR-S15# | (TG)20 | TGCTATCTCCTGCTTGGACA | 60.2 | GCCTCACACACACACACACA | 59.4 | 268 | - | | FAFUR-S16# | (TG)19 | TGCTTGCTAGCTTGGCACTA | 60.4 | ACAACTAGGCCATCAGTGGG | 59.7 | 268 | - | | FAFUR-S19# | (TG)19 | AGCCCAACAGAAATACGCAC | 60.6 | GGGCTCACTCAAAAACCAAA | 58.8 | 269 | - | 引物名称
Primer name | 重复基序
Motif | 左引物序列
Left-primer (5°-3°) | 退火温度
Tm (℃) | 右引物序列
Right-primer (5°-3°) | 退火温度
Tm (℃) | 产物大小
Product size (bp) | PIC | | FAFUR-S20# | (TG)18 | TCGATTGGAGTCTTCAGCAA | 59.9 | CCCATGAGATTGTATTCGGC | 60.3 | 269 | - | | FAFUR-S21# | (TG)18 | TGCACTGTTTAAATTCCCCC | 60.3 | AAATCTCCCTTCATGATGCC | 58.8 | 229 | - | | FAFUR-S25# | (TG)17 | TCGTAGAAGCACTTCAGGGAG | 58.8 | CCAAAATAAGGCCATCGAAA | 60.1 | 162 | - | | FAFUR-S26# | (TG)17 | CTTTGTCCCCTTCTCCATCC | 57.9 | TCTCGAAGTCGCAAGGAACT | 60.5 | 185 | - | | FAFUR-S28# | (TG)16 | TGGCTCACTGAAAATCTCCC | 61.1 | TGTGTGGCAAGATAAGAAGGG | 60.3 | 250 | - | | FAFUR-S29# | (TG)15 | TGCTGATTATGTGCTGCGTC | 59.6 | ATCGATCACACACCTAGGGC | 59.5 | 234 | - | | FAFUR-S46# | (AG)25 | ATCGATCCTGGGGTAGCTTT | 58.4 | TTTCCTCTGCAAGACTGCAA | 58.7 | 262 | - | | FAFUR-S48# | (AG)23 | TTCCAGATTCTTTTCCACGG | 60.3 | GTCACCTGGGAACTACCCCT | 59.6 | 257 | - |
|
|
|