甘蔗栽培种单倍体基因组SSR位点的发掘与应用

王恒波,祁舒婷,陈姝琦,郭晋隆,阙友雄

Development and application of SSR loci in monoploid reference genome of sugarcane cultivar

Heng-Bo WANG,Shu-Ting QI,Shu-Qi CHEN,Jin-Long GUO,You-Xiong QUE

图2 7对不同SSR引物在4个甘蔗属材料上扩增的电泳图 1~4: FAFUR-S44; 5~8: FAFUR-S45; 9~12: FAFUR-S46; 13~16: FAFUR-S47; 17~20: FAFUR-S48; 21~24: FAFUR-S49; 25~28: FAFUR-S50。4个扩增产物为SES208 (1, 5, 9, 13, 17, 21, 25)、LA purple (2, 6, 10, 14, 18, 22, 26)、ROC16 (3, 7, 11, 15, 19, 23, 27)和R570 (4, 8, 12, 16, 20, 24, 28); M: 50 bp DNA ladder (3421A)。

Fig. 2 Electrophoretic patterns of seven pairs of SSR primers amplified in four Saccharum clones 1-4: FAFUR-S44; 5-8: FAFUR-S45; 9-12: FAFUR-S46; 13-16: FAFUR-S47; 17-20: FAFUR-S48; 21-24: FAFUR-S49; 25-28: FAFUR-S50. The amplification products of four samples were SES208 (1, 5, 9, 13, 17, 21, 25), LA purple (2, 6, 10, 14, 18, 22, 26), ROC16 (3, 7, 11, 15, 19, 23, 27), and R570 (4, 8, 12, 16, 20, 24, 28). M: 50 bp DNA ladder (3421A).