亚洲棉短绒突变体纤维发育及其差异基因表达分析

王晓阳,王丽媛,潘兆娥,何守朴,王骁,龚文芳,杜雄明

Analysis of differentially expressed genes and fiber development in Gossypium arboreum fuzzless mutant

Xiao-Yang WANG,Li-Yuan WANG,Zhao-E PAN,Shou-Pu HE,Xiao WANG,Wen-Fang GONG,Xiong-Ming DU

表1 用于荧光定量PCR验证的基因和引物

Table 1 Primers used for real-time quantitative PCR verify of cotton differentially expressed genes (DEGs)

基因编号 Gene 正向引物 Forward primer (5'-3') 反向引物 Reverse primer (5'-3') log2RT-QPCR log2FPKM 功能注释 Functional annotations Ga02G0173 GTGCTCGGAAGAAAACGCTC CGCACCTGCCAAAGTTGTTA 1.31 1.10 谷胱甘肽转移酶 Glutathione S-transferase Ga03G0324 ACAGCAAAAGGCAATCGAGAAC CGTGGGAAGCTATCGGCTTT 2.85 1.65 谷胱甘肽转移酶 Glutathione S-transferase Ga04G0014 CTTTCGGGTCAGGTCGAAGG GTTGTGTTACTCGGGGGACC 3.52 2.89 细胞色素氧化酶 Cytochrome P450 Ga05G3679 GAGCCATCGCTAAGTTCTGGGC TCCAACAATTTCAGGCACGCAA -1.19 -1.40 谷胱甘肽转移酶 Glutathione S-transferase Ga05G3965 CCTGCTATTGCCATCAACACTT TTGTGCTGGACTTGTTCTTGCT -2.00 -1.24 分子伴侣 Molecular chaperone Ga07G0568 CAACGGCGAAAACAACACGGAA TGCACTGGTCTCCTTCTGTGGA 1.31 1.18 甲基转移酶Methyltransferase Ga07G1530 GGTGGTAACCCTAGCTTGTGGC GCGCGTTATGTGTAACTTGCGA 1.26 1.11 转录因子WD40 Ga07G1742 GCCGTGCTAACTTATTCCCTTGC AGCACCCCAAGTAGTAACCAAGC -1.54 -1.20 钙联蛋白 Calnexin 1 Ga07G2088 TTGCATCAGAGCAGCCTGCATA TCTGCTGCCCAAGATTTGCCAT -1.54 -1.32 转录因子 MYB108 Ga07G2611 GCAGAGGCAGCTTTAAGTTTGGG GCATCCCACCAGGCCTAAGTTC -1.61 -1.55 网状蛋白 Reticulon-like protein Ga08G0121 TGTCTTCGGAACCCGGAGATGA GGCATCCCACTAGCAGCATTGA 4.47 6.69 蛋白受体激酶 Receptor-like protein kinase Ga09G0409 TACCGGTGCACTGATCGACCTA TTCTGAGTCGACTGTTCGTCGC 2.03 1.55 功能未知 Unknown Ga09G0435 TGCAGGGTTGTTAAGGTGTGGG TGATTTCGTTATCGGTCCGCCC 1.14 1.05 假定蛋白 Predicted protein Ga09G0661 CGCCATGCTTGCCATCTTCATC CACTGAAGGCAGCTTGATCCGA 1.28 1.31 还原酶 Dihydroflavonol-4-reductase Ga10G0810 ATGAGCCGCCCTATTGACTGCC TGGTAGATGCCTTCGTTGCTGA 2.24 2.51 RNA加工 RNA processing Ga10G1419 CCAACAAGGGCCCAAGTGCTAT TCTTGTTCCCTTGGCCGACTTG -1.22 -1.20 NADH脱氢酶 NADH dehydrogenase Ga11G2832 TAAGATCGGTTCGGCTTCGG ATGCTCCACCCTAACTTGCC 2.01 2.45 假定蛋白 Hypothetical protein Ga11G2833 CACTTCTCGTGTGATGACCTGT CATCTTCTAACGCTGGAATCTG 3.70 5.20 谷胱甘肽转移酶 Glutathione S-transferase Ga11G3625 ATGCTTCTCAACTGCGCAGGAA GCTGTTGTCGAACTTTATGGGGC 2.45 2.86 谷胱甘肽转移酶 Glutathione S-transferase Ga11G3670 GACCGATGAAGAGGAACCCAGC CCATGTGGTGTCCCGGTATGAC -2.88 -1.78 NADH脱氢酶 NADH dehydrogenase Ga12G0113 TCGACGTCGCCCTGTTTTTCAT CTGACGTGGACGCCATGTTGAT 1.53 1.58 假定蛋白 Hypothetical protein Ga10G0013 TACCAGTTGAAGCCGAAGGCTG CCATTGTAAGGCCTGCGCTTTG 2.18 1.81 甲基转移酶N-methyltransferase