利用WGCNA鉴定棉花抗黄萎病相关基因共表达网络
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傅明川,李浩,陈义珍,柳展基,刘任重,王立国
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Identification of co-expressed modules of cotton genes responding to Verticillium dahliae infection by WGCNA
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Ming-Chuan FU,Hao LI,Yi-Zhen CHEN,Zhan-Ji LIU,Ren-Zhong LIU,Li-Guo WANG
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表1 qRT-PCR所用引物
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Table 1 Sequences of the primers used in qRT-PCR
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基因 Gene | 正向引物 Forward primer (5°-3°) | 反向引物 Reverse primer (5°-3°) | Gbar_D11G006810 | GGAGCTGTAGGATCATGCTCAGTG | GCCTTGCCATCCAAAATCCAGC | Gbar_A12G027410 | CGGCTTTCATAGGCAAGGTAGGG | AGCGTAACAAATGCCAATGCCG | Gbar_A04G004150 | CGTGCACTTTTCGGTCGTGATG | AGCTAGCCCTCTTGCTATCCCC | Gbar_D10G024280 | GTAGTTAACGCTGAACAACGTT | CCAACCTCCATACTCTTCTTCA | Gbar_A08G011260 | CAGTAATGTGAAGGCGGCCAGA | CAGTGCTCAACGACCTCGTCAT | Gbar_A13G004330 | GCTCTCAACAATGATGGGGTCCT | AGTTCCAGCTTTGCAAGACCGA | Gbar_A12G027680 | AACTCCGGTAAGTGGGTACCGT | AACGGCGGAGATCGAGTTGATG | Gbar_D03G017340 | AAACGAGAGAGAACGGCGAAGG | CTTAACGGCGAGAGTAACGGCA | Gbar_A07G022210 | GGAAATACCCACCGTGCAACCT | GAGCCAAGGGTGAGTGAGACAC | β-actin | GATTCCGTTGTCCAGAAGTCCT | TACGGTCTGCAATACCAGGGA |
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