玉米tasselseed突变体ts12的遗传分析与分子鉴定

秦利萍,董二飞,白洋,周练,任岚扬,张任凤,刘朝显,蔡一林

Genetic analysis and molecular characterization of tasselseed mutant ts12 in maize

Li-Ping QIN,Er-Fei DONG,Yang BAI,Lian ZHOU,Lan-Yang REN,Ren-Feng ZHANG,Chao-Xian LIU,Yi-Lin CAI

附表1 ts12基因定位、候选基因克隆及表达分析引物序列

Supplementary table 1 Primer sequences for ts12 mapping, candidate gene cloning and expression analysis

引物名称 Primer name 正向引物序列 Forward primer sequence (5′-3′) 反向引物序列 Reverse primer sequence (5′-3′) 标记类型 Genetic marker types LM1 CACAGCAAGAGTACAGCATCAG TGATCCCAGTTCTCGATGTAGC SSR LM2 GGAAATGTTTGACGTGACCTGC CACTTGGCAAACCAATCCAACG SSR LM3 AATAGCGTGCTCCCGTGTTATC CTGTCGTTGCTTGCTTCTTCCA SSR LM4 AATGGGATAAAGACCGGATTCT ATAAGCGTTGTAGGACAGGAGC InDel RM1 TCGGAGAGGACACGGTTTAGTA CATGAACATTGGCGAAGCTACC SSR RM2 GCTCCTGTGTGCAACTTGTTAG GCATATGAATCGGTGTTCGGTG SSR RM3 CTGTTCCTTCGCTTTCACAGAA AGTAGCTCTCTAGTTGTGTCCC SSR RM4 GCTGATTTAAGATATAAAGTGCCTCCC CCTGCTGGACCGGAGTAAAG InDel RM5 AGAACGGTGATTTTTGTCTGGT GTTTTGCATGCTCATGTAGACG InDel Ts2-JD ACAGCAGAGTAGAGTAGCACAC GCCATGGGAATGGGATATTTGG Ts2-qPCR GTGGAGAAGATGGAGGAGGTGGT ATTGATTCACAAGCCGATGAGGTT Actin TCACCCTGTGCTGCTGACCG GAACCGTGTGGCTCACACCA