甘蔗Rieske Fe/S蛋白前体基因ScPetC的克隆及表达分析

郑清雷,余陈静,姚坤存,黄宁,阙友雄,凌辉,许莉萍

Cloning and expression analysis of sugarcane Fe/S precursor protein gene ScPetC

Qing-Lei ZHENG,Chen-Jing YU,Kun-Cun YAO,Ning HUANG,You-Xiong QUE,Hui LING,Li-Ping XU

表1 引物序列及用途

Table 1 Primer sequence used in this study

引物名称 Primer name 引物序列 Primer sequence (5°-3°) 用途 Usage ScPetC-F TGGACGCCAGGGAGTGAAC 基因克隆 Gene cloning ScPetC-R TGTAGCAACAACGCCTTCGG ScPetC-QF GTACAACAACCAGGGCAAGG 实时荧光定量PCR qRT-PCR ScPetC-QR CAGGGACAGGAGGCATACAT CAC-F ACAACGTCAGGCAAAGCAAA 内参基因 Reference gene CAC-R AGATCAACTCCACCTCTGCG CUL-F TGCTGAATGTGTTGAGCAGC 内参基因 Reference gene CUL-R TTGTCGCGCTCCAAGTAGTC ScPetC-Subloc-F CGAACGATACTCGAGGTCGACATGGCGACCTCCGCG 亚细胞定位载体 Vector for subcellular localization ScPetC-Subloc-R GCTCACCATACTAGTGGATCCTGCCTTCCACCACGG ScPetC-AD-F CCATGGAGGCCAGTGAATTCATGGCGACCTCCGCGGCGCT 酵母双杂交载体 Vector for Y2H ScPetC-AD-R AGCTCGAGCTCGATGGATCCTGCCTTCCACCACGGGTCCT SrMV-P1-BK-F TGGCCATGGAGGCCGAATTCATGGCAGGAGCATGGAGCAC 酵母双杂交载体 Vector for Y2H SrMV-P1-BK-R CGCTGCAGGTCGACGGATCC AAAATAAGTAATTTCATTGA SCYLV-P0-BK-F TGGCCATGGAGGCCGAATTCATGCTTTTCAACGAATTCTC 酵母双杂交载体 Vector for Y2H SCYLV-P0-BK-R CGCTGCAGGTCGACGGATCCTATATCATGAGAATAGGTGC ScPetC-YN-F TCTGAGGAGGATCTTCCCGGGATGGCGACCTCCGCGGCGCT 双分子荧光互补实验载体 Vector for BiFC ScPetC-YN-R AGGGCATGCCTGCAGGTCGACGTGCCTTCCACCACGGGTCCT SrMV-P1-YC-F TCTAGGAGCTCGGTACCCGGGATGGCAGGAGCATGGAGCAC 双分子荧光互补试验载体 Vector for BiFC SrMV-P1-YC-R ATCGTATGGGTACATACTAGTAAAATAAGTAATTTCATTGA SCYLV-P0-YC-F CTAGGAGCTCGGTACCCGGGATGCTTTTCAACGAATTCTC 双分子荧光互补实验载体 Vector for BiFC SCYLV-P0-YC-R TCGTATGGGTACATACTAGTTATATCATGAGAATAGGTGC