甘蔗Rieske Fe/S蛋白前体基因ScPetC的克隆及表达分析

郑清雷,余陈静,姚坤存,黄宁,阙友雄,凌辉,许莉萍

Cloning and expression analysis of sugarcane Fe/S precursor protein gene ScPetC

Qing-Lei ZHENG,Chen-Jing YU,Kun-Cun YAO,Ning HUANG,You-Xiong QUE,Hui LING,Li-Ping XU

图5 甘蔗ScPetC与其他物种PetC的系统进化树分析与motif预测 图中进化树橘色部分代表细胞色素b6f复合体类, 蓝色部分代表细胞色素bc1复合体类; III组中的红色圆圈标记为ScPetC; 不同颜色的方框代表不同的组(I、II、III、IV、V、VI、VII)。NP_001237648.2: 大豆; XP_015647138.1: 水稻; XP_0202013301.1: 小麦; Q9ZR03.1: 拟南芥; P26291: 豌豆; AAD55565.1: 团藻; CAA27194: 类红球细菌; CAA45705: 本氏烟; CAA70823: 席藻; CAB72244: 鱼腥藻; CAM57108.1: 半夏; CAM57109.1: 马蹄莲; O49078: 贝母; P05417: 脱氮副球菌; P08067: 酿酒酵母; P08980: 菠菜; P14698: 蓝藻; P23136: 深红红螺菌; P26290: 集胞藻; P26291: 豌豆; P26292: 聚球藻; P37841: 马铃薯; P49728: 衣藻; Q9ZR03: 拟南芥; XP_002441121: 高粱; ACG28308: 玉米; XP_004951312: 小米。

Fig. 5 Phylogenetic tree analysis of sugarcane ScPetC and PetC in other species and their corresponding motif prediction The orange part of the phylogenetic tree represents the cytochrome b6f complex cluster, the blue part represents the cytochrome bc1 complex cluster; the red cycle is labeled as ScPetC; the different color boxes in the figure represent different groups (I, II, III, IV, V, VI, VII). NP_001237648.2: Glycine max; XP_015647138.1: Oryza sativa; XP_0202013301.1: Triticum aestivum; Q9ZR03.1: Arabidopsis thaliana; P26291: Pisum sativum; AAD55565.1: Volvox carteri f nagariensis; CAA27194: Rhodobacter sphaeroides; CAA45705: Nicotiana tabacum; CAA70823: Phormidium laminosum; CAB72244: Trichormus variabilis; CAM57108.1: Pinellia ternata; CAM57109.1: Zantedeschia aethiopica; O49078: Fritillaria agrestis; P05417: Paracoccus denitrificans; P08067: Saccharomyces cerevisiae; P08980: Spinacia oleracea; P14698: Desmonostoc sp. PCC; P23136: Rhodospirillum rubrum; P26290: Synechocystis sp. PCC; P26291: Pisum sativum; P26292: Synechococcus sp. PCC; P37841: Solanum tuberosum; P49728: Chlamydomonas reinhardtii; Q9ZR03: Arabidopsis thaliana; XP_002441121: Sorghum bicolor; ACG28308: Zea mays; XP_004951312: Setaria italica. motif 1: X6NAXCTHLGCVVPX3ENKFXCPCHGSX10GPAP; motif 2: NLX11PDX3RX3NLX22PX3GX3GXXAKGXXGNDX6LX7LXXGLKGDPTYX2V; motif 3: X7WXETDFRTX3PWW; motif 4: XGDX3 GWFCPCHGSHYDX2GRIRXGPAPXNLX15; motif 5: X7MX4DX2AX5 VX7GX6 WXGKPVFXRXRX4IX5VX4LXDX10; motif 6: X2AXSIX ADXV; motif 7: XWLX3GXCTHLGCX; motif 8: X9QLX10; motif 9: LVVEXDXT; motif 10: FX5FX3DDSSX2RSXSPSLXSXFLX3RGFS SNSVSPAHX2GLVXDLPXTVAAIKNPXSKIVYDX2 NHERYPPGDPSKRAFAYFVLTGGRFVY.