甘蔗Rieske Fe/S蛋白前体基因ScPetC的克隆及表达分析

郑清雷,余陈静,姚坤存,黄宁,阙友雄,凌辉,许莉萍

Cloning and expression analysis of sugarcane Fe/S precursor protein gene ScPetC

Qing-Lei ZHENG,Chen-Jing YU,Kun-Cun YAO,Ning HUANG,You-Xiong QUE,Hui LING,Li-Ping XU

图6 甘蔗ScPetC亚细胞定位结果 农杆菌介导ScPetC及空载体在本氏烟叶片瞬时表达48 h后的亚细胞定位结果; 本氏烟叶片表皮细胞被用于明场、黄色荧光、叶绿体自发荧光、明场和黄色及红色荧光叠加后的图像分析; 蓝色箭头1、2分别表示细胞核、质膜; 红色虚线框表示被放大区域; 比例尺=50 μm; 35S::YFP: 携带空载pCAMBIA2300-YFP的农杆菌菌株; 35S:: ScPetC::YFP: 携带重组载体pCAMBIA2300-ScPetC-YFP的农杆菌菌株。

Fig. 6 Results of subcellular localization of sugarcane ScPetC Subcellular localizations of the Agrobacterium tumefaciens mediated transformation of ScPetC and empty vector in Nicotiana benthamiana leaves after 48 h infiltration; the epidermal cells of Nicotiana benthamiana were used for taking images of visible light, yellow fluorescence, chloroplast autofluorescence, and merged visible light. Blue arrows 1 and 2 indicate nucleus and plasma membrane. The area in the red rectangle is magnified. Bar=50 μm; 35S::YFP: the Agrobacterium tumefaciens strain carrying the empty vector pCAMBIA2300-YFP; 35S:: ScPetC::YFP: the Agrobacterium tumefaciens strain carrying the recombinant vector pCAMBIA2300-ScPetC-YFP.