甘蔗Rieske Fe/S蛋白前体基因ScPetC的克隆及表达分析

郑清雷,余陈静,姚坤存,黄宁,阙友雄,凌辉,许莉萍

Cloning and expression analysis of sugarcane Fe/S precursor protein gene ScPetC

Qing-Lei ZHENG,Chen-Jing YU,Kun-Cun YAO,Ning HUANG,You-Xiong QUE,Hui LING,Li-Ping XU

图7 甘蔗ScPetC与高粱花叶病毒和甘蔗黄叶病毒蛋白的互作实验验证 A: 甘蔗ScPetC与SrMV-P1蛋白的酵母双杂交互作验证。B: 甘蔗ScPetC与SCYLV-P0蛋白的酵母双杂交互作验证; SD/-Ade/-His/Leu/-Trp: 腺嘌呤、组氨酸、亮氨酸和色氨酸营养缺陷型平板培养基; SD/-Ade/-His/Leu/-Trp/X-α-Gal: 腺嘌呤、组氨酸、亮氨酸和色氨酸营养缺陷型平板培养基(添加5-溴-4-氯-3-吲哚-α-D-半乳糖苷); SD/-Ade/-His/Leu/-Trp/X-α-Gal/AbA: 腺嘌呤、组氨酸、亮氨酸和色氨酸营养缺陷型平板培养基(添加5-溴-4-氯-3-吲哚-α-D-半乳糖苷和金担子素A)。C: ScPetC与SCYLV-P0蛋白的双分子荧光互补实验; YC、YN、YN-ScPetC和YC-P0分别代表质粒pCAMBIA2300S-YC、pCAMBIA2300S-YN、pCAMBIA2300S-YN-ScPetC和pCAMBIA2300S-YC-P0 [42]。

Fig. 7 The interaction assay of sugarcane ScPetC with virus proteins of Sorghum mosaic virus (SrMV) or Sugarcane yellow leaf virus (SCYLV) A: ScPetC was verified by yeast double-hybrid interaction with SrMV-P1 protein. B: ScPetC was verified by yeast double-hybrid interaction with SCYLV-P0 protein; SD/-Ade/-His/Leu/-Trp: synthetic dropout medium plate without adenine, histidine, leucine, tryptophan; SD/-Ade/-His/Leu/-Trp/X-α-Gal: synthetic dropout medium plate without adenine, histidine, leucine, tryptophan (plus 5-bromo-4-chloro-3- indoxyl-α-D-galactopyranoside); SD/-Ade/-His/Leu/-Trp/X-α-Gal/AbA: synthetic dropout medium plate without adenine, histidine, leucine, tryptophan (plus 5-bromo-4-chloro-3-indoxyl-α-D-galactopyranoside and aureobasidin A). C: the bimolecular fluorescence complementation (BiFC) assay of the interaction between ScPetC and SCYLV-P0 protein; YC, YN, YN-ScPetC, YC-P0 represent the plasmids pCAMBIA2300S-YC, pCAMBIA2300S-YN, pCAMBIA2300S-YN-ScPetC, pCAMBIA2300S-YC-P0, respectively[42].