江苏和东北粳稻稻米理化特性及Wx和OsSSIIa基因序列分析
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赵春芳,岳红亮,田铮,顾明超,赵凌,赵庆勇,朱镇,陈涛,周丽慧,姚姝,梁文化,路凯,张亚东,王才林
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Physicochemical properties and sequence analysis of Wx and OsSSIIa genes in japonica rice cultivars from Jiangsu province and northeast of China
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Chun-Fang ZHAO,Hong-Liang YUE,Zheng TIAN,Ming-Chao GU,Ling ZHAO,Qing-Yong ZHAO,Zhen ZHU,Tao CHEN,Li-Hui ZHOU,Shu YAO,Wen-Hua LIANG,Kai LU,Ya-Dong ZHANG,Cai-Lin WANG
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表2 试验所用引物的序列信息
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Table 2 Sequence information of primers used in this study
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引物名称
Primer | 引物序列
Primer sequence (5°-3°) | 片段长度
Fragment length (bp) | | Wx-seq1 | F: GTGCCACCGTCGCAGGAGAAGAGATGT | 1753 | | R: GGGGTGGTGGTGTGGGTGGCTATTTGT | | | Wx-seq2 | F: GGCTTCACGCAACGGCGCTACAAATAG | 1812 | | R: CCATTCGCCTGCAAAGAACACAAGAAC | | | Wx-seq3 | F: TCGTCGCTGACCGTCGTCGTCTTCAAC | 2327 | | R: CGCAAGCACAGGGCTGGAGAAATCAAC | | | Wx-seq4 | F: ACGGAACGGTATACAATTTCCATCTAT | 1096 | | R: ACATAAATTCAGGGTCCGATAAGATAC | | | OsSSIIa-seq1 | F: CGCGCACACACTCAAACCC | 1573 | | R: ATCTAACAGCTGGAGTAGCCTT | | | OsSSIIa-seq2 | F: CAGGCCCATACAATTAGGGAT | 1426 | | R: TGTCCCTGCTCAATAATTACCAT | | | OsSSIIa-seq3 | F: AGTTCAGCAATTTCTCCGTTT | 1614 | | R: TCACATCCATTCAGACACGAG | | | Wx-mp-O | F: ATGTTGTGTTCTTGTGTTCTTTGCAGGC | 439/292/200 | | R: GTAGATCTTCTCACCGGTCTTTCCCCAA | | | Wx-mp-I | F: GGGTGAGGTTTTTCCATTGCTACAATCG | | | R: GTCGATGAACACACGGTCGACTCAAT | |
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