高粱种子对萌发温度的响应分析与耐低温萌发能力鉴定
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张瑞栋,肖梦颖,徐晓雪,姜冰,邢艺凡,陈小飞,李邦,艾雪莹,周宇飞,黄瑞冬
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Responses of sorghum hybrids to germination temperatures and identification of low temperature resistance
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Rui-Dong ZHANG,Meng-Ying XIAO,Xiao-Xue XU,Bing JIANG,Yi-Fan XING,Xiao-Fei CHEN,Bang LI,Xue-Ying AI,Yu-Fei ZHOU,Rui-Dong HUANG
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表2 不同温度下高粱品种萌发指标的差异比较
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Table 2 Comparison of germination indicators of sorghum under different temperatures
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品种 Hybrid | 温度 Temperature (℃) | 发芽势 Germination potential (%) | 发芽率 Germination percent (%) | 芽长 Bud length (cm) | 根长 Root length (cm) | 芽重 Bud FW (mg) | 根重 Root FW (mg) | S1 | 25 | 78.67±6.11 | 84.90±4.29 | 9.90±0.93 | 13.17±2.38 | 92.00±7.77 | 72.17±7.96 | 20 | 73.33±6.11 | 84.67±2.31 | 4.92±0.62 | 12.80±1.65 | 54.50±10.48 | 76.17±11.89 | 16 | 33.67±4.51 | 82.33±6.03 | 3.35±0.31 | 6.60±0.88 | 25.00±3.10 | 27.50±3.02 | 12 | 0.00±0.00 | 74.67±12.86 | 0.32±0.04 | 1.19±0.17 | 1.83±0.26 | 8.17±0.75 | S2 | 25 | 81.33±2.31 | 84.67±2.52 | 10.37±1.51 | 9.00±1.46 | 89.67±13.17 | 25.00±4.94 | 20 | 80.57±3.44 | 84.33±4.04 | 6.02±0.90 | 15.42±2.80 | 62.50±6.12 | 64.03±12.69 | 16 | 63.33±3.06 | 80.00±2.00 | 2.02±0.16 | 7.52±0.72 | 20.60±3.83 | 63.20±9.45 | 12 | 0.00±0.00 | 72.00±4.00 | 0.42±0.04 | 1.45±0.14 | 2.50±0.46 | 11.50±1.52 | S3 | 25 | 68.00±4.00 | 89.33±2.31 | 9.03±0.65 | 13.60±2.05 | 75.00±8.29 | 74.33±14.62 | 20 | 65.33±7.02 | 88.00±3.46 | 5.77±0.64 | 12.52±1.42 | 57.17±5.91 | 65.67±9.58 | 16 | 21.33±1.53 | 87.33±3.06 | 1.98±0.21 | 6.85±0.90 | 22.00±2.90 | 37.50±5.99 | 12 | 0.00±0.00 | 84.00±6.93 | 0.37±0.08 | 1.40±0.13 | 2.33±0.29 | 10.67±1.21 | S4 | 25 | 60.00±4.00 | 78.33±6.03 | 9.47±1.16 | 6.62±0.66 | 70.67±8.82 | 17.17±1.47 | 20 | 58.67±2.31 | 76.00±5.29 | 5.18±0.73 | 10.18±0.83 | 55.17±4.58 | 57.67±9.58 | 16 | 37.33±2.31 | 68.23±3.28 | 1.86±0.14 | 4.92±0.68 | 21.80±4.23 | 44.80±8.86 | 12 | 0.00±0.00 | 38.67±5.03 | 0.40±0.06 | 1.08±0.12 | 1.50±0.41 | 6.50±0.55 | S5 | 25 | 72.00±4.00 | 74.00±4.00 | 11.53±1.20 | 14.47±1.98 | 106.17±15.78 | 64.67±7.17 | 20 | 31.33±1.53 | 66.67±2.31 | 4.40±0.46 | 7.83±1.16 | 49.50±5.24 | 40.00±6.63 | 16 | 0.00±0.00 | 61.33±8.33 | 2.10±0.29 | 4.62±0.45 | 24.00±4.15 | 26.33±3.27 | 12 | 0.00±0.00 | 10.67±4.62 | 0.10±0.01 | 0.72±0.13 | 0.48±0.15 | 3.33±0.52 | S6 | 25 | 93.33±4.62 | 94.67±2.31 | 10.97±0.96 | 14.80±2.23 | 102.00±14.64 | 59.33±11.45 | 20 | 84.00±5.29 | 93.67±2.08 | 5.10±0.28 | 10.58±1.66 | 64.83±8.47 | 56.00±4.43 | 16 | 22.67±2.31 | 93.33±4.62 | 2.47±0.16 | 5.67±0.75 | 28.67±4.18 | 27.83±4.22 | 12 | 0.00±0.00 | 81.33±4.62 | 0.27±0.05 | 1.27±0.16 | 2.00±0.52 | 8.33±1.03 | S7 | 25 | 86.00±4.00 | 95.33±1.15 | 11.80±0.98 | 9.83±1.05 | 108.58±14.56 | 19.17±3.13 | 20 | 81.33±8.33 | 94.67±1.53 | 6.68±0.26 | 11.73±2.10 | 72.67±10.89 | 52.50±7.82 | 16 | 80.00±4.00 | 94.33±3.21 | 2.28±0.21 | 6.72±1.12 | 22.20±2.79 | 27.40±5.00 | 12 | 0.00±0.00 | 88.00±4.00 | 0.63±0.12 | 2.40±0.42 | 4.17±0.75 | 12.00±0.89 | S8 | 25 | 97.33±2.31 | 98.67±2.31 | 11.13±1.41 | 8.23±1.37 | 77.50±15.14 | 21.17±3.37 | 20 | 92.23±5.08 | 95.67±3.21 | 5.77±0.71 | 13.78±1.50 | 46.50±5.68 | 55.83±7.65 | 16 | 82.00±3.46 | 92.67±3.06 | 1.92±0.07 | 6.26±0.66 | 15.20±1.60 | 31.40±3.72 | 12 | 0.00±0.00 | 90.67±2.31 | 0.40±0.06 | 1.65±0.10 | 1.17±0.41 | 7.00±0.89 | S9 | 25 | 84.43±5.10 | 93.33±2.31 | 9.10±0.94 | 13.65±1.96 | 99.00±14.76 | 38.67±4.59 | 20 | 68.90±6.97 | 93.33±2.31 | 8.70±1.28 | 15.02±1.99 | 75.67±6.98 | 64.83±5.27 | 16 | 18.67±1.53 | 92.00±4.00 | 2.40±0.20 | 6.35±0.86 | 32.17±4.92 | 40.83±6.74 | 12 | 0.00±0.00 | 90.00±2.00 | 0.67±0.08 | 1.93±0.23 | 7.83±1.33 | 14.33±2.42 | S10 | 25 | 73.33±5.03 | 90.67±2.31 | 11.45±0.98 | 13.88±2.72 | 85.33±10.19 | 62.67±3.61 | 20 | 53.33±3.06 | 89.33±2.31 | 5.72±0.74 | 11.58±2.17 | 52.33±6.56 | 56.17±8.35 | 16 | 33.33±4.16 | 86.67±4.62 | 2.65±0.26 | 6.98±1.05 | 27.17±4.75 | 28.83±0.75 | 12 | 0.00±0.00 | 58.67±8.33 | 0.17±0.05 | 1.27±0.23 | 1.33±0.39 | 7.00±1.10 | S11 | 25 | 61.33±6.11 | 85.33±2.31 | 8.92±0.77 | 7.70±1.00 | 81.83±6.21 | 33.33±4.32 | 20 | 58.00±6.00 | 82.67±2.31 | 5.60±0.49 | 10.67±1.35 | 54.50±6.66 | 61.50±10.56 | 16 | 12.33±1.53 | 80.00±4.00 | 2.45±0.19 | 5.30±0.83 | 24.67±3.67 | 30.17±3.60 | S11 | 12 | 0.00±0.00 | 78.67±2.31 | 0.35±0.08 | 2.05±0.18 | 1.33±0.34 | 11.67±1.63 | S12 | 25 | 84.00±2.43 | 89.33±2.31 | 10.13±0.90 | 14.78±2.05 | 111.00±18.22 | 50.00±7.95 | 20 | 61.33±5.13 | 86.67±2.31 | 6.98±1.01 | 9.73±1.41 | 82.33±8.24 | 56.00±10.08 | 16 | 27.33±2.31 | 84.00±4.00 | 2.10±0.26 | 5.67±0.81 | 27.33±4.13 | 36.00±5.73 | 12 | 0.00±0.00 | 81.33±2.31 | 0.68±0.10 | 2.10±0.22 | 6.17±1.17 | 13.33±2.07 | S13 | 25 | 87.33±5.03 | 89.33±3.06 | 8.97±1.34 | 13.22±2.28 | 78.17±13.47 | 44.50±6.09 | 20 | 78.00±5.29 | 84.67±3.06 | 5.87±0.48 | 11.52±2.09 | 59.33±9.93 | 66.67±3.33 | 16 | 5.33±0.58 | 82.67±2.31 | 2.03±0.24 | 5.20±0.89 | 24.50±4.32 | 38.67±3.39 | 12 | 0.00±0.00 | 52.00±4.00 | 0.10±0.00 | 1.02±0.19 | 0.55±0.26 | 6.50±1.05 | S14 | 25 | 82.00±4.00 | 93.33±2.31 | 11.22±1.39 | 14.00±1.52 | 112.17±16.86 | 56.67±9.27 | 20 | 73.33±6.11 | 90.67±2.31 | 6.18±0.39 | 10.88±1.19 | 76.33±9.97 | 60.17±11.32 | 16 | 46.00±4.00 | 80.00±3.46 | 2.17±0.19 | 6.60±0.54 | 25.00±2.53 | 33.17±3.06 | 12 | 0.00±0.00 | 62.00±2.00 | 0.38±0.12 | 1.97±0.27 | 3.17±0.88 | 11.33±2.16 | S15 | 25 | 90.67+2.31 | 93.33±2.31 | 9.37±0.81 | 14.48±1.23 | 76.67±4.59 | 52.83±6.91 | 20 | 89.33+2.31 | 90.67±2.31 | 5.83±0.66 | 13.25±1.46 | 58.33±10.13 | 64.67±8.36 | 16 | 16.00±1.00 | 90.67±6.11 | 2.13±0.15 | 6.68±1.02 | 25.33±4.72 | 33.67±5.65 | 12 | 0.00±0.00 | 70.67±4.62 | 0.27±0.08 | 1.17±0.20 | 2.17±0.75 | 7.17±0.75 | S16 | 25 | 74.67±8.32 | 89.33±2.31 | 9.33±1.05 | 14.80±2.21 | 88.44±6.62 | 51.44±9.39 | 20 | 72.00±9.17 | 81.33±6.11 | 5.25±0.33 | 14.20±2.62 | 52.17±5.56 | 67.67±6.53 | 16 | 42.67±5.03 | 80.67±7.57 | 1.68±0.12 | 8.93±1.28 | 17.67±1.86 | 47.33±7.97 | 12 | 0.00±0.00 | 56.00±4.00 | 0.45±0.10 | 2.10±0.25 | 2.50±0.49 | 12.17±0.75 | S17 | 25 | 78.67±6.11 | 86.67±2.31 | 12.42±0.69 | 9.92±1.21 | 101.67±12.86 | 27.33±4.03 | 20 | 73.23±5.85 | 82.67±4.38 | 7.22±0.63 | 14.75±1.88 | 72.50±11.13 | 75.5±12.63 | 16 | 58.90±3.44 | 78.67±2.31 | 2.14±0.34 | 5.96±0.79 | 21.40±4.03 | 54.40±8.45 | 12 | 0.00±0.00 | 58.00±6.00 | 0.50±0.13 | 1.43±0.16 | 3.33±1.03 | 9.00±1.55 | S18 | 25 | 78.67±8.33 | 89.33±2.31 | 11.83±1.11 | 13.78±2.15 | 108.67±12.14 | 62.50±9.46 | 20 | 62.67±6.11 | 88.00±4.00 | 5.53±0.38 | 9.50±1.37 | 62.00±7.01 | 56.50±9.73 | 16 | 14.67±1.53 | 81.33±6.11 | 2.38±0.36 | 6.55±0.27 | 32.17±6.21 | 36.33±5.20 | 12 | 0.00±0.00 | 68.00±6.93 | 0.43±0.05 | 1.55±0.19 | 2.83±0.75 | 9.50±1.87 | S19 | 25 | 63.33±5.03 | 85.33±4.62 | 8.28±0.54 | 12.95±2.42 | 74.67±11.52 | 50.33±5.09 | 20 | 56.00±8.00 | 72.67±5.03 | 7.50±0.93 | 15.45±2.27 | 77.17±12.91 | 67.67±8.94 | 16 | 17.33±1.53 | 66.00±5.29 | 2.07±0.26 | 6.60±1.25 | 21.00±3.03 | 31.50±6.09 | 12 | 0.00±0.00 | 62.00±6.00 | 0.25±0.05 | 1.53±0.12 | 1.83±0.68 | 7.33±1.03 | S20 | 25 | 86.67±6.65 | 88.00±8.00 | 9.28±1.25 | 15.87±2.83 | 98.33±7.60 | 32.67±1.32 | 20 | 66.00±7.21 | 80.00±4.00 | 6.40±0.92 | 15.38±2.63 | 78.00±7.62 | 82.00±10.71 | 16 | 40.00±5.29 | 77.33±3.06 | 1.97±0.14 | 6.95±1.09 | 24.00±2.28 | 35.33±4.13 | 12 | 0.00±0.00 | 74.00±2.00 | 0.40±0.06 | 2.37±0.35 | 3.83±1.03 | 15.50±1.87 | S21 | 25 | 86.67±6.11 | 94.67±2.31 | 9.27±1.04 | 7.70±1.04 | 87.28±9.61 | 27.33±4.63 | 20 | 80.80±3.73 | 93.33±4.62 | 5.98±0.67 | 12.83±1.96 | 58.00±11.24 | 60.65±7.38 | 16 | 72.67±5.03 | 90.67±6.11 | 2.50±0.28 | 7.12±0.44 | 26.20±3.19 | 56.47±5.18 | 12 | 0.00±0.00 | 89.33±2.31 | 0.30±0.06 | 1.32±0.12 | 2.33±0.45 | 6.50±1.05 | S22 | 25 | 54.67±8.08 | 69.33±4.62 | 11.02±1.43 | 14.17±2.24 | 102.00±18.19 | 100.67±16.85 | 20 | 36.67±3.06 | 66.67±6.11 | 6.20±0.46 | 10.97±1.65 | 67.33±7.00 | 68.83±6.52 | 16 | 8.00±1.00 | 65.33±2.31 | 1.57±0.20 | 4.58±0.52 | 16.17±1.83 | 27.67±2.07 | S22 | 12 | 0.00±0.00 | 61.33±8.33 | 0.23±0.05 | 1.52±0.16 | 1.17±0.27 | 9.00±0.63 | S23 | 25 | 91.33±4.16 | 96.67±1.15 | 9.95±1.34 | 15.23±2.92 | 73.00±10.77 | 46.33±6.92 | 20 | 90.67±4.62 | 96.00±4.00 | 5.40±0.25 | 12.43±1.26 | 45.83±3.82 | 58.17±4.88 | 16 | 13.33±1.53 | 94.67±2.31 | 2.48±0.44 | 6.85±1.07 | 40.67±6.19 | 33.83±6.18 | 12 | 0.00±0.00 | 52.00±8.00 | 0.22±0.04 | 0.95±0.15 | 3.67±0.82 | 5.50±0.55 | S24 | 25 | 76.67±5.03 | 85.33±2.31 | 9.83±1.14 | 8.75±1.49 | 79.50±12.60 | 40.50±7.69 | 20 | 66.67±6.11 | 81.33±5.77 | 5.68±0.62 | 7.72±1.26 | 54.50±7.37 | 40.67±7.34 | 16 | 16.00±1.73 | 77.33±8.33 | 2.05±0.21 | 5.67±0.48 | 25.83±2.48 | 24.33±4.68 | 12 | 0.00±0.00 | 54.00±2.00 | 0.23±0.05 | 1.13±0.22 | 1.17±0.41 | 7.50±1.05 | S25 | 25 | 68.33±2.52 | 89.33±2.31 | 10.90±0.51 | 16.05±2.61 | 89.67±7.55 | 84.67±9.14 | 20 | 64.00±6.00 | 89.33±6.11 | 6.22±0.58 | 14.17±1.87 | 64.50±6.44 | 98.50±18.25 | 16 | 60.00±3.46 | 86.67±6.11 | 2.68±0.19 | 7.68±0.61 | 33.00±2.53 | 46.17±5.81 | 12 | 0.00±0.00 | 57.33±4.62 | 0.15±0.05 | 1.25±0.08 | 1.58±0.49 | 8.83±1.17 | S26 | 25 | 89.33±8.33 | 92.00±4.00 | 9.35±1.13 | 16.28±3.11 | 74.67±11.2 | 67.33±11.93 | 20 | 65.33±6.43 | 90.67±6.11 | 4.85±0.71 | 10.35±1.23 | 48.00±9.23 | 59.00±5.59 | 16 | 36.00±1.73 | 89.33±2.31 | 2.62±0.30 | 6.18±0.73 | 27.00±3.41 | 32.33±6.02 | 12 | 0.00±0.00 | 77.33±8.33 | 0.13±0.05 | 0.95±0.08 | 0.67±0.22 | 6.83±0.75 | S27 | 25 | 90.67±2.31 | 93.33±2.31 | 8.30±1.08 | 15.13±2.06 | 95.17±11.34 | 51.00±9.84 | 20 | 89.33±2.31 | 89.33±6.11 | 7.35±1.08 | 16.13±2.32 | 98.50±7.92 | 78.17±8.66 | 16 | 62.00±6.00 | 88.67±3.06 | 2.27±0.20 | 8.07±0.79 | 28.67±3.93 | 46.00±7.40 | 12 | 0.00±0.00 | 38.00±2.00 | 0.55±0.05 | 2.28±0.29 | 3.50±0.84 | 12.17±1.47 | S28 | 25 | 84.00±10.58 | 88.00±6.93 | 11.87±1.37 | 16.00±1.11 | 94.83±15.46 | 61.33±4.72 | 20 | 60.67±6.11 | 86.67±6.11 | 6.20±0.87 | 12.07±2.01 | 68.67±10.78 | 67.67±11.31 | 16 | 36.00±2.00 | 85.33±6.11 | 2.63±0.30 | 6.83±1.04 | 30.83±5.12 | 35.50±6.12 | 12 | 0.00±0.00 | 78.67±2.31 | 0.38±0.10 | 1.93±0.34 | 2.83±0.71 | 11.00±1.67 | S29 | 25 | 82.67±4.16 | 88.00±4.00 | 10.73±1.54 | 8.62±1.40 | 80.83±10.32 | 32.67±4.80 | 20 | 80.57±3.44 | 84.00±6.93 | 5.15±0.34 | 10.85±1.03 | 39.33±4.89 | 47.83±7.47 | 16 | 73.33±2.31 | 82.67±4.62 | 1.76±0.14 | 6.44±0.83 | 15.20±2.48 | 35.20±3.19 | 12 | 0.00±0.00 | 66.67±6.11 | 0.25±0.05 | 1.65±0.27 | 1.33±0.41 | 8.33±1.03 | S30 | 25 | 83.33±7.02 | 86.67±6.11 | 9.40±0.73 | 9.62±1.06 | 93.00±8.10 | 20.67±3.78 | 20 | 78.30±5.00 | 85.33±8.33 | 6.25±0.86 | 16.78±2.67 | 69.17±8.06 | 86.17±16.46 | 16 | 70.00±8.00 | 84.00±4.00 | 2.16±0.24 | 7.94±1.04 | 23.00±3.90 | 45.20±7.08 | 12 | 0.00±0.00 | 56.00±4.00 | 0.57±0.08 | 1.93±0.37 | 5.67±1.21 | 14.33±1.63 | 品种 Hybrid(H) | F-value | 42.97 | 42.84 | 7.28 | 16.59 | 16.3 | 24.31 | P-value | ** | ** | ** | ** | ** | ** | 处理Treatment(T) | F value | 3919.17 | 397.94 | 7772.28 | 2495.72 | 4756.06 | 2039.14 | P-value | ** | ** | ** | ** | ** | ** | H×T | F value | 17.42 | 7.62 | 8.09 | 10.44 | 7.08 | 21.35 | P-value | ** | ** | ** | ** | ** | ** | 变异系数CV (%) | 74.29 | 17.34 | 82.17 | 60.62 | 78.02 | 59.88 |
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