蓖麻种子结构的解剖和显微观察
Anatomy and microscopic observation of Ricinus communis seed structure
A~C: 体视显微镜图片, 示种阜的形态; D~J: 横切面; K和L: 纵切面; D和H~J: 希夫试剂染色, 示细胞壁由糖类物质构成; E~G、K、L: 番红和固绿双重染色, 示细胞壁不角质层和含木质素。A: 种阜背面观, 示种阜中部的浅沟将种阜上部分为2个球形突起; B: 种阜腹面观, 示种阜和外种皮相连接; C: 种阜底面观, 示种阜管道在内种皮内层和外层之间的开口(箭头), 该开口靠近种子背侧, 以及白色的内种皮外层; D: 种阜上部横切面, 示2个球形突起; E: 种阜浅沟基部横切, 示未出现种阜管道; F: 种阜中部外侧横切面, 示外侧3层小型的细胞和内部大型的细胞; G: 种阜中部横切放大图, 示发达的纹孔(箭头); H: 种阜中部横切面, 示出现种阜管道; I: 图H中方框a放大图, 示Y字形种阜管道; J: 图H中方框b放大图, 示种阜中的维管束(箭头); K: 距种阜管道中轴一点距离的种阜纵切面, 示种阜管道(箭头)上端并未和浅沟基部联通; L: 沿种阜管道中轴的纵切面, 示种阜管道基部的开口(箭头)。EM: 外种皮; CC: 种阜管道。
A-C: stereomicrograph, showing the shape of the caruncle; D-J: transection; K and L: longitudinal section; D and H-J: images stained with Schiff’s reagent, showing cell walls composed of carbohydrates; E-G, K, L: images with double staining of saffron and fixed green, showing cell walls without lignin and cuticle. A: view on the back of the caruncle, showing the shallow ditch in the middle part of the caruncle, which divided its upper part into two spherical protrusions; B: ventral view of the caruncle, showing the tight combination of caruncle and episperm; C: view on the bottom of the caruncle, showing an opening (arrow) of caruncle channel between the inner and outer layers of the endopleura and near the dorsal surface of the seed, and the outer layer of the white endopleura; D: transection of the upper part of the caruncle, showing two spherical protrusions; E: transection of shallow ditch base of the caruncle, showing no caruncle channel; F: transection of the middle lateral side of the caruncle, showing three layers of small and cuticle-free cells on the outside and large parenchyma cells inside; G: detailed view of transection of the middle part of the caruncle, showing developed pits (arrow); H: transection of middle part of the caruncle, showing the caruncle channel; I: detailed view of box a in figure H, showing Y-shaped caruncle channel; J: detailed view of box b in figure H, showing vascular bundle in the caruncle; K: longitudinal section of the caruncle a short distance from the central axis of the caruncle channel, showing the upper end of the caruncle channel (arrow) which did not connected with the base of the shallow ditch; L: longitudinal section along the central axis of the caruncle channel, showing the opening (arrow) at the base of the caruncle channel. EM: episperm; CC: caruncle channel.
