大豆GmNRT1.2a和GmNRT1.2b基因的克隆及功能探究

李国纪,朱林,曹金山,王幼宁

Cloning and functional analysis of GmNRT1.2a and GmNRT1.2b in soybean

Guo-Ji LI,Lin ZHU,Jin-Shan CAO,You-Ning WANG

图1 GmNRT1.2a和GmNRT1.2b过表达载体构建 A: PCR扩增后得到的GmNRT1.2a和GmNRT1.2b目的基因片段; B: pEGAD过表达载体图谱及酶切位点。

Fig. 1 Construction of GmNRT1.2a and GmNRT1.2b overexpression vectors A: the coding sequence of GmNRT1.2a and GmNRT1.2b were obtained through PCR amplification. B: the partial vector map of pEGAD.