大豆GmNRT1.2a和GmNRT1.2b基因的克隆及功能探究

李国纪,朱林,曹金山,王幼宁

Cloning and functional analysis of GmNRT1.2a and GmNRT1.2b in soybean

Guo-Ji LI,Lin ZHU,Jin-Shan CAO,You-Ning WANG

图3 GmNRT1.2a和GmNRT1.2b在不同浓度下的硝酸盐下的表达模式 大豆在0N (0 mmol L-1)、LN (0.25 mmol L-1)和HN (15.75 mmol L-1) 3种硝酸盐浓度梯度下生长到15 d, 取大豆根部组织样品。用荧光定量PCR检测GmNRT1.2a (A)和GmNRT1.2b (B)的表达, GmELF1b为内参基因。图中标以不同字母的基因表达水平在P < 0.05时差异显著。

Fig. 3 Expression pattern of GmNRT1.2a and GmNRT1.2b under different concentrations of nitrate Soybeans were germinated in vermiculite at different nitrate concentrations (0N-0 mmol L-1, LN-0.25 mmol L-1, HN-15.75 mmol L-1). The roots were collected at 15 days after germination. The expression of GmNRT1.2a (A) and GmNRT1.2b (B) was analyzed by RT-qPCR. Bars with different lowercase letters in each figure are significantly different at P < 0.05.