大豆GmNRT1.2a和GmNRT1.2b基因的克隆及功能探究

李国纪,朱林,曹金山,王幼宁

Cloning and functional analysis of GmNRT1.2a and GmNRT1.2b in soybean

Guo-Ji LI,Lin ZHU,Jin-Shan CAO,You-Ning WANG

图5 在低氮培养条件下过表达GmNRT1.2a或GmNRT1.2b增加结瘤数目 A, B: 在接种根瘤菌28 d后, 利用荧光定量PCR检测EV、35S::GmNRT1.2a和35S::GmNRT1.2b单条毛状根表达, 以GmELF1b基因作为内参基因。标以不同小写字母的柱值在不同转基因大豆植株间在P < 0.05时的差异显著。C: EV、35S::GmNRT1.2a和35S::GmNRT1.2b的单条根的根瘤表型, Bar = 5 mm。D: 统计EV、35S::GmNRT1.2a和35S::GmNRT1.2b的根瘤数目。*和***分别表示转基因株系35S::GmNRT1.2a和35S::GmNRT1.2b的根瘤数目与对照在P < 0.05和P < 0.001水平差异显著。

Fig. 5 Nodule number increased by 35S::GmNRT1.2a or 35S::GmNRT1.2b under low nitrate condition A, B: RT-qPCR analysis of GmNRT1.2a or GmNRT1.2b in single hairy root transformed with empty vector, 35S::GmNRT1.2a or 35S::GmNRT1.2b inoculated with Bradyrhizobium japonicum USDA110 at 10 and 28 days treatment. GmELF1b was used as an endogenous control for gene expression. Bars with different lowercase letters in each figure are significantly different at P < 0.05. C: the phenotype of nodule per hairy root transformed with empty vector (EV), 35S::GmNRT1.2a and 35S::GmNRT1.2b at 28 DAI. Bar = 5 mm. D: nodule number per hairy root transformed with empty vector (EV), 35S::GmNRT1.2a or 35S::GmNRT1.2b were counted at 28 DAI. The values followed by * and *** are significantly different at P < 0.05 and P < 0.001.