甘薯蔗糖转运蛋白基因IbSUT3的克隆及功能分析

王丹丹,柳洪鹃,王红霞,张鹏,史春余

Cloning and functional analysis of the sweet potato sucrose transporter IbSUT3

Dan-Dan WANG,Hong-Juan LIU,Hong-Xia WANG,Peng ZHANG,Chun-Yu SHI

表1 引物信息

Table 1 Information of primers

引物Primer 序列 Sequence (5'-3') 目的 Purpose E3-F AGATAGTAATGGTGGCCTCCATTGC EST序列扩增 E3-R GCGAAGCCAATGAGGAAGACAGC EST sequence amplification Sp-F1 AGATAGTAATGGTGGCGTCCATTGC 3'端和5'端RACE扩增 Sp-F2 GCGTCCTTTATGTGGCTTTGTGG 3' and 5'-RACE amplification Sp-R1 TGAGATTGGCGCAGTAAACG Sp-R2 GCGGCGAAGCCAATGAGGAA IbSUT3-F CCATTCAGGCTGCGCAACT IbSUT3全长扩增 IbSUT3-R TTAAGAAATTAATTCCAAGGTCCAT IbSUT3 full-length amplification SUT3Q-F CGGAAAATCCTCCTTGCAAGTG 荧光定量PCR SUT3Q-R GACGCCACCATTACTATCTTCC Real-time PCR Actin-F CTGGTGTTATGGTTGGGATGG Actin-R GGGGTGCCTCGGTAAGAAG SUT3m-F CATCGATATGGAGAGAGACTCCGTTAACG 酵母功能验证 SUT3m-R CCCCGGGTCAGTGGAAACCACCACCAGAT Yeast function verification SUT3g-F ACGCGTCGACATGGAGAGAGACTCCGTTAACGGAA 亚细胞定位 SUT3g-R GGACTAGTACGTGGAAACCACCACCAGATAGCTCA Subcellular localization