基于CRISPR/Cas9核糖核蛋白体DNA定点内切酶体外活性建立高效基因型分析技术
王南,祁显涛,刘昌林,谢传晓,朱金洁

Establishment of an efficient genotyping technique based on targeted DNA endonuclease in vitro activity of CRISPR/Cas9 ribonucleoprotein
Nan WANG,Xian-Tao QI,Chang-Lin LIU,Chuan-Xiao XIE,Jin-Jie ZHU
图1 Cas9与Cas9NG蛋白的原核表达及纯化
A: 原核表达载体结构示意图; B: Cas9纯化蛋白10% SDS-PAGE胶图; C: Cas9-NG纯化蛋白10% SDS-PAGE胶图。M: Protein marker; 1: 上清液中蛋白; 2: Ni柱纯化蛋白; 3: 阳离子交换柱纯化蛋白。
Fig. 1 Purification of Cas9 and Cas9NG protein
A: Schematics diagram of protein expression vector; B: 10% SDS-PAGE of purified Cas9; C: 10% SDS-PAGE of purified Cas9NG. M: Protein marker. 1: Supernatant protein; 2: Ni-column crude purified protein; 3: Cation exchange column purification protein.