基于黄麻转录组序列SNP位点的CAPS标记开发与验证

陶爱芬,游梓翊,徐建堂,林荔辉,张立武,祁建民,方平平

Development and verification of CAPS markers based on SNPs from transcriptome of jute (Corchorus L.)

Ai-Fen TAO,Zi-Yi YOU,Jian-Tang XU,Li-Hui LIN,Li-Wu ZHANG,Jian-Min QI,Ping-Ping FANG

表3 39对SNP引物的编号、名称及序列

Table 3 Name and sequence of 39 pairs of SNP primers

引物名称 Primer name 正向引物序列 Forward primer sequence (5′-3′) 反向引物序列 Reverse primer sequence (5′-3′) 4CL SNP1-1 GTGCTTTTCATCGGTGGTTT TGTCGTAAAACTGCTGCTGG 4CL SNP1-2 CGATTTTCATCTCGGCATTT GCCACCCATAGTTTTGGCTA 4CL SNP2-1 AGCTCCCATGAAAGCAAAGA AGCTCCCATGAAAGCAAAGA 4CL SNP2-2 AGCTCCCATGAAAGCAAAGA CGATTTTCATCTCGGCATTT 4CL SNP3-1 GAGCTTAGCGCGAGAAGTGT TGTCGTAAAACTGCTGCTGG 4CL SNP3-2 TCTCGGCATTTCTAACCACC GCCACCCATAGTTTTGGCTA COMT SNP1F1 CACCGTTTTCCGTTGTTCTT GTTGGAAAGCTAGCCAAACG COMT SNP1F2 ACACTCCATCTTGGTTTGGC GCTACCCATGGCGTTAAAAA COMT SNP1F3 CCAGGTTTTCAACACTGCAA GCATCTCCATTGGGAACACT COMT SNP1F4 TGAAGATGACAATAAAGCGAAAA GTGGACAATCAGTGTGTGGC COMT SNP1F5 ATGCAAGAAAGCCGAGTTTG GCAGGATTCCAAGGTTTCAA COMT SNP2F1 AAGGTGATAGAAACACGCCG GTTGGAAAGCTAGCCAAACG COMT SNP2F2 ACTCCATCTTGGTTTGGCAC GCTACCCATGGCGTTAAAAA 引物名称 Primer name 正向引物序列 Forward primer sequence (5′-3′) 反向引物序列 Reverse primer sequence (5′-3′) COMT SNP2F3 GCTTAACCGGGTTGTTGATG GCATCTCCATTGGGAACACT COMT SNP2F4 TGAAGATGACAATAAAGCGAAAA GCATCTCCATTGGGAACACT COMT SNP2F5 ATGCAAGAAAGCCGAGTTTG TTGATTGCATCATGTTGGCT COMT SNP3F1 AATTTCACCGTTTTCCGTTG GTTGGAAAGCTAGCCAAACG COMT SNP3F2 ACTCCATCTTGGTTTGGCAC AAAAACTGCGGTGGAGATTG COMT SNP3F3 GCTTAACCGGGTTGTTGATG CCATTGTCTGGAATGGCTTT COMT SNP3F4 TGAAGATGACAATAAAGCGAAAA TGGACAATCAGTGTGTGGCT COMT SNP3F5 ATGCAAGAAAGCCGAGTTTG CATAATCCGGGTGGAAAAGA MYB SNP F1 TTGCAATGGATTTGTGCAAT GATTCCCAGCAAATGGAAGA MYB SNP F2 TGCCTTGTCCTCTCACTAAGC TTGAGGCCACATGTTCTAATTG MYB SNP F3 CCTTGCTATCATTGCCCATT TTTATGGCCCTCAAAACTGG MYB SNP F4 CAAAAGCTCATCCTCTTCCG CAAACTGCTTGGCTCATCAA MYB SNP F5 AAGTGTTCAAAGAGAAGCAGCA TTATAGATGGCGATGGAGGC MYB SNP F6 AAGCAGAAGCAAAATCCCAA AAGTAGCCATGGAGGTGTGG MYB SNP F7 GGCCATGAGTTTCAACGACT GACGGGAAGAGAAAACCCTT MYB SNP F8 CTGCGTTTGTAACCCCAGAT GCTTCCTCTTCTGCTTCTCG MYB SNP F9 CGACTCTTTCGGGACTCAAG TCGTCGGCGTTTAAGAAGTT MYB SNP F10 TTACACCACCGTAACCGACA AGATGTGGATCGGATCAAGG MYB SNP F11 AAGGAAGCCATGGAAGGACT ATGCTTCTACCAATGCCAGG MYB SNP F12 TCGATCATCAGCAACCAAAA GCATTGAATTTTCCGTGGTT MYB SNP F13 CCTGATCTTAAGCGTGGCTC TTTGGTGACGGAGTTGATGA MYB SNP F14 GGCCATTGGAAAACTCAAAA TGGATGAAGAGCCTTTCACA MYB SNP F15 ATCTTTGCCCAAAAATGCTG GCAATTACCTGCTTCCCAAA MYB SNP F16 GAGAAGGGAAATGGCATCAA CTTTGGTGGAACTGGAAGGA MYB SNP F17 TTCTTCAGCTCTCCAACGCT GGCTTGTGGTATTGTGAGCA MYB SNP F18 CCCTTTCTAGCATATGGGCA AACATCCCTCCATATTCATGTGT