基于黄麻转录组序列SNP位点的CAPS标记开发与验证

陶爱芬,游梓翊,徐建堂,林荔辉,张立武,祁建民,方平平

Development and verification of CAPS markers based on SNPs from transcriptome of jute (Corchorus L.)

Ai-Fen TAO,Zi-Yi YOU,Jian-Tang XU,Li-Hui LIN,Li-Wu ZHANG,Jian-Min QI,Ping-Ping FANG

表4 CAPS引物名称、对应的unigene编号及内切酶名称

Table 4 Name, corresponding unigene code, and endonuclease name of CAPS primers

序号 Code	引物名称 Primer name	unigene编号 unigene code	内切酶的名称 Endonuclease name
1	4CL CAPS1-1	unigene_17597	Ssp I
2	4CL CAPS2-1	unigene_17597	Ssp I
3	4CL CAPS3-1	unigene_17597	Ssp I
4	COMT CAPS1-1	unigene_04800	Cla I
5	COMT CAPS1-3	unigene_09002	Bsi YI
6	COMT CAPS1-5	unigene_08713	Ava III
7	COMT CAPS2-1	unigene_04800	Cla I
8	COMT CAPS2-3	unigene_09002	Bsi YI
9	COMT CAPS2-5	unigene_08713	Ava III
10	COMT CAPS3-1	unigene_04800	Cla I
11	COMT CAPS3-3	unigene_09002	Bsi YI
12	COMT CAPS3-5	unigene_08713	Ava III
13	MYB CAPS1	unigene_47261	Psi I
14	MYB CAPS2	unigene_17018	Mse I
序号 Code	引物名称 Primer name	unigene编号 unigene code	内切酶的名称 Endonuclease name
15	MYB CAPS3	unigene_53709	Mbo II
16	MYB CAPS5	unigene_64377	Mnl I
17	MYB CAPS6	unigene_10508	Mbo I
18	MYB CAPS7	unigene_21631	Mfe I
19	MYB CAPS8	unigene_31305	Sec I
20	MYB CAPS9	unigene_16840	Hpy99 I
21	MYB CAPS11	unigene_02399	Alu I
22	MYB CAPS12	unigene_02162	Asu I
23	MYB CAPS13	unigene_46122	Sec I
24	MYB CAPS14	unigene_10460	Tsp RI
25	MYB CAPS17	unigene_59879	Rsa I
26	MYB CAPS18	unigene_49512	Mae I