BnaBZR1和BnaPIF4基因调控甘蓝型油菜弱光光效的机制

冯韬,谭晖,官梅,官春云

Mechanism of BnaBZR1 and BnaPIF4 regulating photosynthetic efficiency in oilseed rape (Brassica napus L.) under poor light

Tao FENG,Hui TAN,Mei GUAN,Chun-Yun GUAN

表1 基因克隆引物

Table 1 Primer for gene cloning

引物 Primer 克隆产物 Product of cloning 序列 Sequence (5'-3') PIF4_mF mRNA of BnaPIF4 ATAGATCTCATCCCTAAAGA PIF4_mR TATGTTCAAAAGATAGCCTTAG BZR1_mF mRAN of BnaBZR1 GGAGAAGGAAAGAGAGATTC BZR1_mR TTGAGAGAAACAAAATGGGC PIF4_cF CDS of BnaPIF4 ATGGAACACCAAGGTTGGAG PIF4_cR CTAACGGGGACCGTCGG BZR1_cF CDS of BnaBZR1 ATGACGTCAGATGGAGCTACG BZR1_cR TCAACCACGAGCTTTGCC PIF4_pF Promoter of BnaPIF4 ATTGAAACCGATTGTAAGGA PIF4_pR AGAAACAAAGGAGCATAAAG BZR1_pF Promoter of BnaBZR1 GGTTATTTTCAAATAATGGATG BZR1_pR CTTGAGCTCTTAGCCCTGTG PIF4_uF 5'-UTR of BnaPIF4 ATAGATCTCATCCCTAAAGA PIF4_uR GTCAGATCTCAGATTTGGAAAGC PIF4_dF Full-length gene of BnaPIF4 ATAGATCTCATCCCTAAAGAAG PIF4_dR TTTTGACAAACTAAACCAGG