BnaBZR1和BnaPIF4基因调控甘蓝型油菜弱光光效的机制

冯韬,谭晖,官梅,官春云

Mechanism of BnaBZR1 and BnaPIF4 regulating photosynthetic efficiency in oilseed rape (Brassica napus L.) under poor light

Tao FENG,Hui TAN,Mei GUAN,Chun-Yun GUAN

图4 BnaPIF4可变剪接对基因表达和翻译的影响 A: XY881和XY883中BnaPIF4时空表达; B: 弱光和2,4-BL处理下XY881和XY883中BnaPIF4表达; C: 含不同5'-UTRs重组pRI101-AN 载体结构; D: 转基因拟南芥中BnaPIF4 mRNA和蛋白表达; E: 转BnaPIF4拟南芥表型; F: 转BnaPIF4拟南芥RuBPCase含量。DAG表示种子萌发后天数。

Fig. 4 Alternative splicing affects BnaPIF4 gene expression and protein translation A: temporal and spatial gene expression of BnaPIF4 in XY881 and XY883; B: the expression of BnaPIF4 in XY881 and XY883 under poor light stress and 2,4-BL treatment; C: construction of pRI101-AN vector with different 5'-UTRs; D: the mRNA expression of BnaPIF4 and the protein expression of BnaPIF4; E: the phenotype of transgenic BnaPIF4 Arabidopsis thaliana; F: the RuBPCase protein content of transgenic BnaPIF4 Arabidopsis thaliana. DAG means days after seed germination.