利用CRISPR/Cas9技术编辑水稻温敏不育基因TMS5

陈日荣,周延彪,王黛君,赵新辉,唐晓丹,许世冲,唐倩莹,符星学,王凯,刘选明,杨远柱

CRISPR/Cas9-mediated editing of the thermo-sensitive genic male-sterile gene TMS5 in rice

Ri-Rong CHEN,Yan-Biao ZHOU,Dai-Jun WANG,Xin-Hui ZHAO,Xiao-Dan TANG,Shi-Chong XU,Qian-Ying TANG,Xing-Xue FU,Kai WANG,Xuan-Ming LIU,Yuan-Zhu YANG

表1 本研究所用的引物

Table 1 Primer sequences used in this study

引物名称 Primer name 引物序列 Primer sequence (5′-3′) TMS5-target-F TGGCCAGCGGCAAGTCATCGCCGG TMS5-target-R AAACCCGGCGATGACTTGCCGCTG M13F GTAAAACGACGGCCAG GUS-F CGTCCGTCCTGTAGAAACCC GUS-R GTGCGGATTCACCACTTGC TMS5-CX-F TCCAACGCATAGCAGTAGTCG TMS5-CX-R TGCCATCGTATCTCCGGTAAA Cas9-F ACCGAGGGAATGAGAAAGCC Cas9-R CCTTCTGGGTGGTCTGGTTC HPT-F GAAGTGCTTGACATTGGGAGT HPT-R AGATGTTGGCGACCTCGTATT