利用CRISPR/Cas9技术编辑水稻温敏不育基因TMS5

陈日荣,周延彪,王黛君,赵新辉,唐晓丹,许世冲,唐倩莹,符星学,王凯,刘选明,杨远柱

CRISPR/Cas9-mediated editing of the thermo-sensitive genic male-sterile gene TMS5 in rice

Ri-Rong CHEN,Yan-Biao ZHOU,Dai-Jun WANG,Xin-Hui ZHAO,Xiao-Dan TANG,Shi-Chong XU,Qian-Ying TANG,Xing-Xue FU,Kai WANG,Xuan-Ming LIU,Yuan-Zhu YANG

图1 CRISPR/Cas9-TMS5载体构建示意图 A: TMS5靶点位置。红色字母为靶点序列, 蓝色字母为PAM序列。B: pCAMBIA1301-Cas9-TMS5-gRNA重组载体结构示意图。LB: 左边界; RB: 右边界。

Fig. 1 Schematic diagram of the CRISPR/Cas9-TMS5 vector construction A: Schematic diagram of the targeted site in TMS5. The red letters are the target genome sequences. The blue letters are the protospacer adjacent motif (PAM) sequences. B: Schematic diagram of the pCAMBIA1301-Cas9-TMS5-gRNA construct. LB: left border; RB: right border.