利用CRISPR/Cas9技术编辑水稻温敏不育基因TMS5

陈日荣,周延彪,王黛君,赵新辉,唐晓丹,许世冲,唐倩莹,符星学,王凯,刘选明,杨远柱

CRISPR/Cas9-mediated editing of the thermo-sensitive genic male-sterile gene TMS5 in rice

Ri-Rong CHEN,Yan-Biao ZHOU,Dai-Jun WANG,Xin-Hui ZHAO,Xiao-Dan TANG,Shi-Chong XU,Qian-Ying TANG,Xing-Xue FU,Kai WANG,Xuan-Ming LIU,Yuan-Zhu YANG

图2 T0代转基因植株的鉴定 ZG75(A)、CYG(B)、YG0618(C)、ZG07(D)、T0361(E)、7679(F)、2537(G)、6150(H)、6379(I)、1109(J)阳性转基因植株的PCR鉴定(上)和GUS组织化学染色(下)。M: DNA marker; 1~10: 转基因植株。

Fig. 2 Identification of the T0 transgenic plants Identification of the positive transgenic plants by PCR (upper) and GUS histochemical staining (lower) in ZG75(A), CYG(B), YG0618(C), ZG07(D), T0361(E), 7679(F), 2537(G), 6150(H), 6379(I), and 1109(J). M: DNA marker; 1-10: transgenic plants.