利用CRISPR/Cas9技术编辑水稻温敏不育基因TMS5

陈日荣,周延彪,王黛君,赵新辉,唐晓丹,许世冲,唐倩莹,符星学,王凯,刘选明,杨远柱

CRISPR/Cas9-mediated editing of the thermo-sensitive genic male-sterile gene TMS5 in rice

Ri-Rong CHEN,Yan-Biao ZHOU,Dai-Jun WANG,Xin-Hui ZHAO,Xiao-Dan TANG,Shi-Chong XU,Qian-Ying TANG,Xing-Xue FU,Kai WANG,Xuan-Ming LIU,Yuan-Zhu YANG

图3 T0代纯合突变类型 红色字母为靶点序列; 蓝色字母为PAM序列; 绿色字母为插入的碱基; 横线表示碱基缺失; +表示插入; -表示缺失; WT表示野生型。

Fig. 3 Homozygous mutation types of T0 generation The red letters are the target genome sequences; the blue letters are PAM; the green letters are the insert base; the horizontal line indicates the missing base; +: insertion; -: deletion; WT: wild-type.