利用CRISPR/Cas9技术编辑水稻温敏不育基因TMS5

陈日荣,周延彪,王黛君,赵新辉,唐晓丹,许世冲,唐倩莹,符星学,王凯,刘选明,杨远柱

CRISPR/Cas9-mediated editing of the thermo-sensitive genic male-sterile gene TMS5 in rice

Ri-Rong CHEN,Yan-Biao ZHOU,Dai-Jun WANG,Xin-Hui ZHAO,Xiao-Dan TANG,Shi-Chong XU,Qian-Ying TANG,Xing-Xue FU,Kai WANG,Xuan-Ming LIU,Yuan-Zhu YANG

附图1 PCR鉴定T1代无选择标记基因的突变株 ZG75(A)、CYG(B)、YG0618(C)、ZG07(D)、T0361(E)、7679(F)、2537(G)、6150(H)、6379(I)、1109(J)非转基因植株的PCR鉴定。M: DNA marker; WT: 野生型; 1: 阳性对照; 2~6: GUS染色不呈蓝色的T1代植株。

Supplementary Fig. 1 Identification of the marker-free T1 transgenic plants by PCR Identification of the marker-free transgenic plants by PCR in ZG75(A), CYG(B), YG0618(C), ZG07(D), T0361(E), 7679(F), 2537(G), 6150(H), 6379(I), 1109(J). M: DNA marker; WT: wild-type; 1: positive control; 2-6: the leaf of T1 plants did not show blue by GUS staining.